Generation, concentration and efficient transfer of VSV-G pseudo

Details Generation, concentration and efficient transfer of VSV-G pseudo Generation, concentration and efficient transfer of VSV-G pseudo

1993-08-10

1996-04-30

Stone, Jacqueline M.

Chemistry: molecular biology and microbiology

Vector, per se

424 932, 435239, 935 32, C12N 1586, C12N 702, C12N 1547, C12N 1548

Patent

active

055124216

ABSTRACT:
The present application discloses retrovirus-derived vectors in which the retroviral envelope glycoprotein has been replaced by the G glycoprotein of vesicular stomatitis virus, and the use of these vectors in the transfer of exogenous genes into the cells of a wide variety of non-mammalian organisms. Also disclosed is a method for the generation of retroviral vectors in high titers, wherein a recombinant, stable host cell line is provided which harbors the retroviral vector of interest without envelope protein. High-titer retroviral vector production is initiated by introducing nucleic acid encoding a functional membrane-associated protein into the cell line. The vectors disclosed in the present application can be concentrated by ultracentrifugation to titers greater than 10.sup.9 cfu/ml which are especially useful in human gene therapy trials, and can also infect cells, such as hamster and fish cells, that are ordinarily resistant to infection with vectors containing the retroviral envelope protein.

REFERENCES:
J. C. Burns et al. (1993) Proc. Natl. Acad. Sci. USA 90: 8033-8037.
N. Hopkins (1993) Proc. Natl. Acad. Sci. USA 90:8759-8760.
J.-K. Yee et al. (1994) Methods in Cell Biology 43:99-112.
J.-K. Yee et al. (1994) Proc. Natl. Acad. Sci. USA 91:9564-9568.
Graham et al. Virology, 52:456-457 (1973).
Jolly et al. Mol. Cell Biol., 6:1141-1145 (1986).
Li et al. Virology, 171:331-341 (1989).
Littlefield et al. Nature, 211:250-252 (1966).
Miller et al. Mol. Cell Biol., 5:431-437 (1985).
Miller et al. Science, 225:630-632 (1984).
Okayama et al. Mol Cell Biol. 3:280-289 (1983).
Quade et al. Virology, 98:461-465 (1979).
Rose et al. Cell, 30:753-762 (1982).
Rose et al. J. Virol., 39:519-528 (1981).
Vaux et al. Nature, 336:36-42 (1988).
Adam et al. J. Virol. 62(10):3802-3806 (1988).
Ban et al. J. Gen. Virol., 70:1987-1993 (1989).
Baskin, Yvonne The Gene Doctors, Medical Genetics at the Frontier, William Morrow & Co. N.Y. p. 211 (1984).
Bender et al. J. Virology, 61:1639-1646 (1987).
Besmer et al. J. Virol., 21(3):965-973 (1977).
Cone, Robert et al. Proc. Ntl. Acad. Sci. USA 81:6349-6353 (1988).
Danos et al. Proc Natl. Acad. Sci, USA 85:6460-6464 (1988).
Delouis et al. Biochem. Biophys. Res. Comm. 169(1):8-14 (1990).
Embretson et al. J. Virology 60(2):662-668 (1986).
Embretson et al. J. Virology 61:2675-2683 (1987).
Hoshino et al. Int. J. Cancer, 36:671-675 (1985).
Huang, A. S. et al. J. Virology, 12:659-662 (1973).
Livingston et al. Virology, 70:432-4390 (1976).
Love et al. Virology, 57:217-278 (1974).
Mann et al. Cell, 33:153-159 (1983).
Markowitz et al. J. Virology, 62(4):1120-1124 (1988).
Markowitz et al. Virology, 167:400-406 (1988).
Miller et al. Human Gene Therapy, 1:5-15 (1990).
Miller et al. Mol. Cell Biol, 6(8):2895-2902 (1986).
Mohr et al. Virology, 117:522-529 (1982).
Morgenstern et al. Nucl. Acids Res., 18:3587-3596 (1990).
Schlegel et al. Cell, 32:639-646 (1983).
Schnitzer et al. J. Virology 23(3):449-454 (1977).
Sorge et al. Mol. Cell Biol, 4(9):1730-1737 (1984).
Tato et al. Virology 88:71-81 (1978).
Watanabe et al. Mol. Cell Biol. 3: 2241-2249 (1983).
Watanabe et al. Proc. Natl. Acad. Sci., USA 79-5986-5990 (1982).
Weiss et al. Virology, 100:252-274 (1980).
Whitt et al. J. Virology, 63:3569-3578 (1989).
Willey et al. J. Virology 62:139-147 (1988).
Wills et al. J. Cell Biol. 99:2011-2023 (1984).
Wilson et al. J. Virology, 63:2374-2378 (1989).
Witte et al. Cell, 11:505-511 (1977).
Zavada, J. J. Gen Virol., 125:183-191 (1972).
Zavada et al. Acta Virol., 27:110-118 (1983).
N. Emi et al. J. Cell. Biochem. Suppl, 14 A:367, Abstract D408.
J. W. Wills Nature 340:323-324 (1989).
J. R. McLachlin et al. Prog. Nucl. Acid Res. and Mol. Biol. 38:91-134 (1990).
L. M. Roman et al. Experimental Cell Research 175:376-387 (1988).
Young et al., "Efficient Incorporation of Human CD4 Protein into Avian Leukosis Virus Particles," Science, 250:1421-1423 (1990).

Affiliated with

Also associated with

Rating

Generation, concentration and efficient transfer of VSV-G pseudo does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Generation, concentration and efficient transfer of VSV-G pseudo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Generation, concentration and efficient transfer of VSV-G pseudo will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-627623