Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1990-07-26
1993-03-30
Lacey, David L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 4352573, 4353201, C12N 1563, C12N 1509, C12P 2100
Patent
active
051983460
ABSTRACT:
Novel DNA-binding proteins, especially repressors of gene expression, are obtained by variegation of genes encoding known binding proteins and selection for proteins binding the desired target DNA sequence. A novel selection vector may be used to reduce artifacts. Heterooligomeric proteins which bind to a target DNA sequence which need not be palindromic are obtained by a variety of methods, e.g., variegation to obtain proteins binding symmetrized forms of the half-targets and heterodimerization to obtain a protein binding the entire asymmetric target.
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Guterman Sonia K.
Kent Rachel B.
Ladner Robert C.
Ley Arthur C.
Cooper Iver P.
Lacey David L.
Protein Engineering Corp.
Ulm John D.
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