Generating vascular smooth muscle cells in vitro from ES cells

Details Generating vascular smooth muscle cells in vitro from ES cells Generating vascular smooth muscle cells in vitro from ES cells

2008-04-08

2008-04-08

Crouch, Deborah (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

C435S325000

Reexamination Certificate

active

07354763

ABSTRACT:
A simplified and inexpensive method for the in-vitro identification, isolation and culture of human vasculogenic progenitor cells is provided. The method and the progenitor cells provided herein can be used for in-vitro vascular engineering, treatment of congenital and acquired vascular and hematological abnormalities, for evaluation and development of drugs affecting vasculo- and angiogenic processes, and for further investigation into tissue differentiation and development.

REFERENCES:
patent: 2001/0041936 (2001-11-01), Corbitt et al.
patent: 2002/0045258 (2002-04-01), Bickenbach et al.
patent: 2003/0194802 (2003-10-01), Itskovitz-Eldor et al.
patent: WO 00/70021 (2000-11-01), None
patent: WO 01/81549 (2001-01-01), None
Conley et al, The International Journal of Biochemistry and Cell Biology, 36:555-567, 2004.
Odorico et al, Stem Cells, 19:193-204, 2001.
Yancopoulos et al. “Vascular-Specific Growth Factors and Blood Vessel Formation”, Nature 407: 242-248, 2000.
Gittenberger-de Groot et al. “Smooth Muscle Cell Origin and Its Relation to Heterogeneity in Development and Disease”, Arterioscler Thromb. Vasc. Biol. 19: 1589-1594, 1999.
Condorelli et al. “Cardiomyocytes Induce Endothelial Cells to Transdifferentiate Into Cardiac Muscle: Implications for Myocardium Regeneration”, PNAS, 98(19): 10733-10738, 2001. Claims: 1-78.
Lammert et al. “Induction of Pancreatic Differentiation by Signals from Blood Vessels”, Science 294: 564-567, 2001.
Schuldiner et al. “Effects of Eight Growth Factors on the Differentiation of Cells Derived from Human Embryonic Stem Cells”, PNAS 97(21): 11307-11312, 2000.
Nishikawa et al. “Progressive Lineage Analysis by Cell Sorting and Culture Identifies FLK1+VE-Cadherin+ Cells at a Diverging Point of Endothelial and Hemopoietic Lineages”, Development 125: 1747-1757, 1998.
Itskovitz-Eldor et al. “Differentiation of Human Embryonic Stem Cells into Embroid Bodies Comprising the Three Embrionic Germ Layers”, Molecular Medicine 6(2): 88-95, 2000.
Matsumoto et al. “Liver Organogenesis Promoted by Endothelial Cells Prior to Vascular Function”, Science 294: 559-563, 2001.
Carmeliet “Mechanism of Arteriogenesis”, Nature Medicine 6(3): 389-395, 2000.
Fujimoto et al. “Step-Wise Divergence of Primitive and Definitive Haematopoietic and Endothelial Cell Lineages During Embryonic Stem Cell Differentiation”, Genes Cells 6(12): 1113-1127, 2001. abstract.
Yamashita et al. “FLK1-Positive Cells Derived From Embryonic Stem Cells Serve as Vascular Progenitors”, Nature 408: 92-96, 2000. Claims: X: 11, 17, 18, 24, 25,26. / Y: 1-5, 12-16, 19-23.
Kaufman et al. “Hematopoietic Colony-Forming Cells Derived From Human Embryonic Stem Cells”, PNAS 98(19): 10716-10721, 2001.
Levenberg et al. “Endothelial Cells Derived From Human Embryonic Stem Cells”, PNAS 99(7): 4391-4396, 2002.
Nishikawa “A Complex Linkage in the Development Pathway of Endothelial and Hematopoietic Cells”, Curr Opin Cell Biol. 13(6): 673-8, 2001.
Ogawa et al. “Expression of Alfa4-Integrin Defines the Earliets Precursor of Hematopoietic Cell Lineage Diverged from Endothelial Cells”, Blood 93(4): 1168-1177, 1999.
Pfeifer et al. “Transgenesis by Lentiviral Vectors: Lack of Gene Silencing in Mammalian Embryonic Stem Cells and Preimplantation Embryos”, Proc Natl Acad Sci USA 99(4): 2140-5, 2002.
Magyar et al. “Mass Production of Embryoid Bodies in Microbeads”, Ann NY Acad Sci 944: 135-43, 2001.
Rovira et al. “Stable in Vivo Expression of Glucose-6-Phosphate Dehydrogenase (G6PD) and Rescue of G6PD Deficiency in Stem Cells by Gene Transfer”, Blood 96(13): 4111-7, 2000.
Ishiwata et al. “New Approach for the Establishment of Mouse Early Embryon Stem Cells and Induction of their Differentiation”, Hum Cell 14(4): 283-91, 2001.
Guan et al. “Embryonic Stem Cells in Vitro—Prospects for Cell and Development Biology, Embryotoxicology and Cell Therapy”, Altex 16(3): 135-141, 1999.
Koch et al. “Tissue Engineering with Chondrocytes”, Facial Plast Surg 18(1): 59-68, 2002.
Risbud “Tissue Engineering: Implications in the Treatment of Organ an Tissue Defects”, Biogerontology 2(2): 117-25, 2001.
Lee “Tissue-Engineered Human Living Skin Substitutes: Development and Clinical Application”, Yonsei Med J 41(6): 774-9, 2000.
Atala “Engineering Tissues and Organs”, Curr Opin Urol 9(6): 517-26, 1999.
Mueller-Klieser “Three-Dimensional Cell Cultures: From Molecular Mechanism to Clinical Applications”, Am J Physiol 273(4 Pt 1): C1109-23, 1997.
Maria-Engler et al. “Microencapsulation and Tissue Engineering as an Alternative Treatment of Diabetes”, Braz J Med Biol Res 34(6): 691-7, 2001.
Kawamoto et al. “Therapeutic Potential of Ex Vivo Expanded Endothelial Progenitor Cells for Myocardial Ischemia”, Circulation, 103(5): 634-637, 2001. Claims: 47-70.
Bach et al. “VE-Cadherin Mediates Endothelial Cell Capillary Tube Formation in Fibrin and Collagen Gels”, Experimental Cell Research 238: 324-334, 1998.
Feraud et al. “Embryonic Stem Cell-Derived Embryoid Bodies Development in Collagen Gels Recapitulates Sprouting Angiogenesis”, Laboratory Investigation 81(12): 1669-1681, 2001.
Kehat et al. “Human Embryonic Stem Cells Can Differentiate Into Myocytes With Structural and Functional Properties of Cardiomyocytes”, The Journal of Clinical Investigation 108(3): 407-414, 2001.
Tamborini et al. “c-Kit and c-Kit Ligand (SCF) in Synovial Sarcoma (SS): an mRNA Expression Analysis in 23 Cases”, British Journal of Cancer 85(3): 405-411, 2001.
Yamamura et al. “Expression of the Smooth Muscle Calponin Gene in Human Osteosarcoma and its Possible Association with Prognosis”, Int. J. Cancer (Pred. Oncol.) 79: 245-250, 1998.
Syed et al. “Differential Expression of Angiopoietin-1 and Angiopoietin-2 in Colon Carcinoma”, Cancer 92: 1138-43, 2001.
Hicklin et al. “Monoclonal Antibody Strategies to Block Angiogenesis”, DDT 6(10): 517, 2001.
Peichev et al. “Expression of VEGFR-2 and AC133 by Circulating Human CD34+ Cells Identifies a Population of Functional Endothelial Precursors”, Blood 95(3): 952-958, 2000.
Reyes et al. “Origin of Endothelial Progenitors in Human Postnatal Bone Marrow”, J. Clin. Invest 109: 337-346, 2002.
Corada et al. “Monoclonal Antibodies Directed to Different Regions of Vascular Endothelial Cadherin Extracellular Domain Affect Adhesion and Clustering of the Protein and Modulate Endothelial Permeability”, Blood 97: 1679-1684, 2001.
10. Assessing Human Stem Cell Safety.
Lumelsky et al. “Differentiation of Embryonic Stem Cells to Insulin-Secreting Structures Similar to Pancreatic Islets”, Science 292(5520): 1389-1394, 2001.
Shamblott et al. “Human Embryonic Germ Cell Derivatives Express a Broad Range of Developmentally Distinct Markers and Proliferate Extensively in Vitro”, PNAS 98(1): 113-118, 2001.
Quarmby et al: “Irradiation Induces Upregulation of CD31 in Human Endothelial Cells”, Artesiosclerosis, Thrombosis, and Vascular Biology, 19(3): 588-597, 1999.
Faloon et al. “Basic Fibroblast Growth Factor Positively Regulates Hematopoietic Development”, Development, 127: 1931-1941, esp. P.1931-1933, 2000. Claims: X: 11, 17, 18, 24, 25, 26. / Y: 1-5, 12-16, 19-23.
Nakayama et al. “Vascular Endothelial Growth Factor Synergistically Enhances Bone Morphogenetic Protein-4-Dependent Lymphohematopoietic Cell Generation From Embryonic Stem Cells In Vitro”, Blood, 95: 2275-2283, 2000. Claims: 1-48, 71-78.
Nishikawa “Embryonic Stem Cells as a Source of Hematopoietic and Vascular Endothelial Cells In Vitro”, Journal of Allergy and Clinical Immunology, 100: S1-2-S1-4, 1997. Claims

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