Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Patent
1983-08-31
1986-07-01
Warden, Robert J.
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
435289, 935 88, 436 89, 436 90, 222137, 525 541, 525 5411, 422 62, 422111, 422116, C12M 100, C12M 136, B67D 552, G05B 1700
Patent
active
045980497
ABSTRACT:
A microprocessor controlled, general purpose gene synthesizer for programmably synthesizing selected nucleotide sequences. Depending upon a programmed chemistry, metered volumes of selected bases and reagent/solvents are sequentially metered into the bottom of a reaction cell containing solid-supported nucleotides, where desired linkages are achieved in an agitated suspension. Self-refilling syringe metering and electrically initiated, pneumatically operated valving insures economical, non-contaminated synthesis. Preprogrammed, selectable chemistries and operator programmed sequences are operatively coupled under microprocessor control to ensure a general purpose synthesizer.
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Itakura Keiichi
Kaplan Bruce E.
Sims Carl W.
Zelinka Richard J.
Haugen Orrin M.
Jay Jeremy
Nikolai Thomas J.
Systec Inc.
Warden Robert J.
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