Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-12-24
1998-08-11
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 911, C12N 922
Patent
active
057926409
ABSTRACT:
The present invention reveals methods for cloning hybrid restriction endonucleases and for enzymatically inactivating a target DNA. The method for cloning hybrid restriction endonucleases involves co-expression of a ligase. A first plasmid contains a gene encoding a DNA ligase. A second plasmid contains a gene encoding a hybrid restriction endonuclease and is compatible with the first plasmid. The method involves transfecting host cells with the first plasmid, so that DNA ligase is produced, followed by transfecting the cells with the second plasmid. The method for enzymatically inactivating a target DNA involves preparing a plasmid, phage, virus or any other delivery vehicle such as a liposome containing a gene encoding a nuclease, delivering the gene into cells, inducing the cells to produce the nuclease and enzymatically inactivating the target DNA.
REFERENCES:
patent: 5200333 (1993-04-01), Wilson
Rima Youil et al., Screening for Mutations by Enzyme Mismatch Cleavage with T4 Endonuclease VII, Proc. Natl. Acad. Sci. USA, Jan. 1995, pp. 87-91, vol. 92.
Alla Lishanski et al., Mutation Detection by Mismatch Binding Protein, MutS, in Amplified DNA: Application to the Cystic Fibrosis Gene, Proc. Natl. Acad. Sci. USA, Mar. 1994, pp. 2674-2678, vol. 91.
Adrian Whitehouse et al., A Carboxy Terminal Domain of the hMSH-2 Gene Product is Sufficient for Binding Specific Mismatched Oligonucleotides, Biochemical and Biophysical Research Communications, 1996, vol. 225, 289-295, Article No. 1168.
Yang-Gyun Kim et al., Hybrid Restriction Enzymes: Zinc Finger Fusions to FOK I Cleavage Domain, Proc. Natl. Acad. Sci. USA, Feb. 1996, 1156-1160, vol. 93.
Yang-Gyun Kim et al., Chimeric Restriction Endonuclease, Proc. Natl. Acad. Sci. USA, Feb. 1994, pp. 883-887, vol. 91.
Lin Li et al., Functional Domains in FOK I Restriction Endonuclease, Proc. Natl. Acad. Sci. USA, May 1992, pp. 4275-4279, vol. 89.
Lin Li et al., C-terminal Deletion Mutants of the FOK I Restriction Endonuclease, Gene, 133 1993, pp. 79-84.
Lin Li et al., Alteration of the Cleavage Distance of FOK Restriction Endonuclease by Insertion Mutagenesis, Pro. Natl. Acad. Sci, USA, Apr. 1993, pp. 2764-2768, vol. 90.
Yang-Gyun Kim et al., Insertion and Deletion Mutants of FOK I Restriction Endonuclease, The Journal of Biological Chemistry, Dec. 16, 1994, pp. 31978-31982, vol. 269, No. 50.
Baohua Huang et al., Splase: A New Class IIS Zinc-Finger Restriction Endonuclease with Specificity for Sp1 Binding Sites, Journal of Protein Chemistry, 1996, pp. 481-489, vol. 15.
Yen Choo et al., In Vivo Repression by a Site-Specific DNA-Binding Protein Designed Against an Oncogenic Sequence, Nature, Dec. 15, 1994, pp. 642-645, vol. 372.
Patterson Jr. Charles L.
The Johns Hopkins University
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