Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Synthesis of peptides
Patent
1995-03-29
1997-06-24
Davenport, Avis M.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Synthesis of peptides
530333, 530300, A61K 3802, C07K 500, C07K 700
Patent
active
056418620
ABSTRACT:
A method to obtain selected individual peptides or families thereof which have a target property and optionally to determine the amino acid sequence of a selected peptide or peptides to permit synthesis in practical quantities is disclosed. In general outline, the method of the invention comprises synthesizing a mixture of randomly or deliberately generated peptides using standard synthesis techniques, but adjusting the individual concentrations of the components of a mixture of sequentially added amino acids according to the coupling constants for each amino acid/amino acid coupling. The subgroup of peptides having the target property can then be selected, and either each peptide isolated and sequenced, or analysis performed on the mixture to permit its composition to be reproduced. Also included in the invention is an efficient method to determine the relevant coupling constants.
REFERENCES:
patent: 4510083 (1985-04-01), Blacklock et al.
patent: 4572894 (1986-02-01), Imahori et al.
patent: 4833092 (1989-05-01), Geysen
patent: 4946778 (1990-08-01), Ladner et al.
patent: 5010175 (1991-04-01), Rutter et al.
patent: 5053454 (1991-10-01), Judd
patent: 5182366 (1993-01-01), Huebner et al.
patent: 5194392 (1993-03-01), Geysen
patent: 5223409 (1993-06-01), Ladner et al.
patent: 5266684 (1993-11-01), Rutter et al.
patent: 5438119 (1995-08-01), Rutter et al.
Berman, J. et al., "Rapid Optimization of Enzyme Substrates Using Defined Substrate Mixtures" J. of Biol. Chem. (1992) 267:1434-1437.
Creighton, T.E. Proteins, Wett. Freeman & Co., 1984, pp. 39-45.
Flynn et al., "Peptide-binding Specificity of the Molecular Chaperone BiP" Nature (1991) 353:726-730.
Furka et al., (1988) 14th Int'l Congress of Biochemistry vol. 5, abstract FR:013.
Furka, A. Sebestyen . . . More Peptides by Less Labor Abstract, 10th Int. Symp. on Med. Chem., Budapest.
Furka, A. et al., "Computer Made Electrophoretin Peptide Maps," 2nd Int. Conf. on Biochem. Symp. Kesythely.
Furka, A. et al., "General Method for Rapid Synthesis of Multicomponent Pep Mix," Int. J. Pep Res 37:487-493.
Furka et al., "Cornucopia of Peptides by Synthesis," Publication date unknown.
Getzoff et al. "Mechanisms of Antibody Binding to a Protein" Science (1987) 235:1191-1196.
Geysen, H.M. et al., "Use of Peptide Synthesis to Probe Viral Antigens for Epitopes to a Resolution of a Single Amino Acid" Proc Natl Acad Sci USA (1984) 81:3998-4002.
Geysen, et al., "Chemistry of Antibody Binding to a Protein" Science (1987) 235:1184-1190.
Houghten et al., "Simultaneous Multiple Peptide Synthesis: The Rapid Preparation of Large Numbers of Discrete Peptides for Biological, Immunological, and Methodological Studies" Biotechniques (1986) 4(6):522-528.
Houghten, R.A., "General Method for the Rapid solid-Phase Synthesis of Large Numbers of Peptides: Specificity of Antigen-Antibody Interaction at the Level of Individual Amino Acids", Proc. Natl. Acad. Sci. USA, (1985), 82:5131-5135.
Houghten et al., "Generation and Use of Synthetic Peptide Combinatorial Libraries for Basic Research and Drug Discovery" Nature (1991) 354:84-86.
Kemp et al., "Rate Constants for Peptide .rho.-Nitrophenyl Ester Coupling Reactions in Dimethylformamide. A Model for Steric Interactions in the Peptide Bond Forming Transition State" J. Org. Chem. (1974) 39(26):3841-3847.
Kovacs, J., "Racemization and Coupling Rates of N.alpha.-Protected Amino Acids and Peptide Active Esters: Predictive Potential" The Peptides: Analysis, Synthesis, Biology (1980) Gross et al. (ed.) 485-539.
Lam et al., "A New Type of Synthetic Peptide Library for Identifying Ligand-Binding Activity" Nature (1991) 354: 82-84.
Lu, Heieng et al., Chem. Abs. 106(1):2453f, 1986.
Merrifield, "Solid Phase Synthesis" Angewandte Chemie, (1985) 24(10): 799-810.
Petithory et al., "A Rapid Method for Determination of Endoproteinase Substrate Specificity: Specificity of the 3C Proteinase from Hepatitis A Virus" Proc. Natl. Acad. Sci. USA (1991) 88:11510-11514.
Ragnarsson et al., "Studies on the Coupling Step in Solid Phase Peptide Synthesis. Further Competition Experiments and Attempts to Assess Formation of Ion Pairs" J. Org. Chem. (1974) 39(26):3837-3841.
Stewart et al., Solid Phase Peptide Synthesis (2nd edition), Gross et al, (ed), Pierce Chemical Company (1984).
Zuckermann et al., "Identification of Highest-Affinity Selection from Equimolar Peptide Mixtures Generated by Robotic Synthesis", Proc. Natl. Acad. Sci. USA (1992) 89:4505-4509.
Rutter William J.
Santi Daniel V.
Bozicevic Karl
Davenport Avis M.
The Regents of the University of California
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