Gene, vector and transformant for thermostable lipase and prepar

Details Gene, vector and transformant for thermostable lipase and prepar Gene, vector and transformant for thermostable lipase and prepar

1991-01-10

1994-04-26

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435198, 435 691, 435 711, 435 712, 4351723, 43525233, 43525234, 4353201, 536 232, 935 6, 935 9, 935 11, 935 14, 935 27, 935 29, 935 56, 935 72, C12N 1555, C12N 1574, C12N 121, C12N 920

Patent

active

053066367

ABSTRACT:
A thermostable lipase gene coding for a thermostable lipase, a vector having this gene, a transformant containing this vector, methods for preparing them and for preparing a thermostable lipase by cultivating the transformant are disclosed. The thermostable lipase gene is obtained from a cell belonging to the bacterial strain Pseudomonas sp. KWI-56 by a technique of genetic engineering. The thermostable lipase gene is inserted into a high expression vector, which is introduced into a cell of a host microorganism to prepare a transformant. By cultivating this transformant, a thermostable lipase can be produced.

REFERENCES:
Kurta Water Co. "Pseudomonas KWI-56 strain-used for the Production . . . " Dewent Biotechnology Abstracts vol. 8(17)113-114 Abstract No. 89-10503.
Kugimiya et al. "Molecular Cloning and Nucleotide Sequence of the Lipase . . . " Biochem. Biophys. Res. Commun. vol. 141(1) 185-190.

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