Gene transfer vector

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435253, 435878, 435320, 935 27, C12N 1500, C12N 120, C12N 100, C12R 141

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active

046861840

ABSTRACT:
A Tn-Mob element is provided comprising a transposon with a Mob-site inserted therein. Vectors and bacterial strains are provided containing the Tn-Mob element, as well as methods for its use. Such methods include the production of plasmid free bacterial strains.

REFERENCES:
Simon, R. et al., in Molecular Genetics of the Bacteria-Plant Interaction, (A. Puhler, ed), pp. 98-106, 1983.
Berg, De., in DNA, Insertion, Elements, Plasmids, and Episomes, (eds. Bukhari, Shapiro, Adhya), pp. 205-212, 1977.
Mobile Genetic Elements (ed. James A. Shapiro), pp. 226, 233, 234, 262 and 266, 1983.
Klee, H. J. et al., J Bacteriol, vol. 150, pp. 327-331, 1982.
Datta, N., et al., (1971), J. Bact, 108:1244-1249.
Datta, N. and R. W. Hedges (1972), J. Gen. Microbiol., 70:453-460.
Olsen, R. H. and P. J. Shipley (1973), J. Bact. 113:772-780.
Beringer, J. G. (1974), J. Gen. Microbiol., 84:188-198.
Starlinger, P. (1980), Plasmid, 3:241-259.
Calos, H. and J. H. Miller (1980), Cell, 20:579-595.
Priefer, U. B., et al., (1980) in Antibiotic Resistance, Transposition and Other Mechanisms (S. Mitshuhashi, L. Rosival and V. Krcmery, eds.), Springer-Verlag, Berlin, pp. 91-96.
Simon. R, (1980) in Antibiotic Resistance, Transposition and Other Mechanisms, (S. Mitshuhashi, L. Rosival and V. Krcmery, eds.), Springer-Verlag, Berlin, pp. 35-42.
Simon, R. et al., (1983), Analysis of Plasmid Borne Genes in Rhizobium meliloti by Tn5 Mutagenesis, Winnipeg Symposia-Proc., 8th N. Amer. Rhizobium Conference, (meeting held in 1981), published Jan. 1983.
8th North American Rhizobium Conference Abstract Book, pp. 1-3, 7-15, 20, 22, 25, 28-29, 42-43, 100-103, 105, 128, 130, 132 and 136 (Aug. 3-7, 1981).
Proceedings, 8th North American Rhizobium Conference, pp. 90-114 (K. W. Clark & J. H. G. Stephens eds. 1983).

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