Gene transfer using herpes virus vectors as a tool for neuroprot

Details Gene transfer using herpes virus vectors as a tool for neuroprot Gene transfer using herpes virus vectors as a tool for neuroprot

1994-08-08

1997-08-26

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Vector, per se

4352351, C12N 1586, C12N 701

Patent

active

056610335

ABSTRACT:
Defective herpes simplex virus (HSV) vectors have been used to express a glucose transporter gene. The vectors were propagated using HSV conditional mutants. The efficacy of this system in vivo was tested by microinjection of the vector into adult rat hippocampus and measurement with [.sup.14 C]2-deoxyglucose uptake autoradiography, as well as measurement of kainic acid-(KA-) induced lesions. The vector significantly enhanced glucose transport and decreased neuronal damage in regions near the site of injection without causing adverse cytopathology. Neuroprotection was conferred by injecting the vector prior to, concurrently with, and following neuronal insult.

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