Gene transfer in chicken bursal stem cells

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S069100, C435S455000, C435S456000, C800S004000, C800S023000

Reexamination Certificate

active

06946266

ABSTRACT:
The invention relates to a method of expressing proteins in higher eukaryotes in vitro. In general, the invention sets forth a method of expressing exogenous proteins as fusion partners with the immunoglobulin molecules of avian species specifically chickens. The technology involves, in one embodiment, retroviral-mediated gene transfer and transplantation of stem cells from the Bursa of Fabricus in chickens.

REFERENCES:
patent: 5470726 (1995-11-01), Miller et al.
patent: 5766945 (1998-06-01), Miller
Neiman et al. A retroviral myc gene induces preneplastic transformation of lymphocytes in a bursal transplanation assay. Proc Natl Acad Sci U S A. Jan. 1985;82(1):222-6.
Thompson et al. The effect of alterations in myc gene expression on B cell development in the bursa of Fabricius. Cell. Nov. 6, 1987;51(3):371-81.
Lee et al. Role of Nr13 in regulation of programmed cell death in the bursa of Fabricius. Genes Dev. Mar. 15, 1999;13(6):718-28.
Database Swiss-Prot entry P06295.
Burns et al., “Vesicular stomatis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells,”Proc. Nat'l Acad. Sci. USA,90:8033-8037, 1993
Eskola and Toivanen, “Effect of in Ovo treatment with cyclophosphamide on lymphoid system in chicken,”Cell Immunology,13:459-471, 1974.
Landau et al., “Packaging system for rapid production of murine leukemia virus vectors with variable tropism,”J. Virol.,66:5110-5113, 1992.
Lee et al., “Role of Nr13 in regulation of programmed cell death in the bursa of fabricius,”Genes&Development, 13:718-728, 1999.
Miller et al., “Cloning of the celluar receptor for amphotropic murine retroviruses reveals homology to that for gibbon ape leukemia virus,”Proc. Nat'l Acad. Sci. USA,91:78-82, 1994.
Miller et al., “Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus,”J. Virol.,65(5):2220-2224, 1991.
Neiman et al., “Induction of apoptosis during normal and neoplastic B-cell development in the bursa of Fabricius,”Proc. Nat'l Acad. Sci. USA,88:5857-5861, 1991.
Thompson et al., “The effect of alterations in myc gene expression on B cell development in the Bursa of fabricius,”Cell,51:371-381, 1987.
Yee et al., “A general method for the generation of high-titer, pantropic retroviral vectors: highly efficient infection of primary heptocytes,”Proc. Nat'l Acad. Sci. USA,91:9564-9568, 1994.
Yom et al., “Genetic engineering of milk composition: modification of milk components in lactating transgenic animals,”Amer. J. Clin. Nutr.,58(suppl):299S-306S, 1993.

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