Chemistry: molecular biology and microbiology – Apparatus – Mutation or genetic engineering apparatus
Patent
1994-11-14
1997-06-24
Stone, Jacqueline M.
Chemistry: molecular biology and microbiology
Apparatus
Mutation or genetic engineering apparatus
4351723, 4352851, 4352891, 4353201, 514 44, C12N 1500, C12N 500, A61K 4200
Patent
active
056416806
ABSTRACT:
A method and apparatus for performing gene therapy treatment. The method comprises selecting a genetic vector, introducing a genetic material into the genetic vector, combining the modified genetic vector with an electronic pulse delivery buffer solution with cells from an organism, and performing electronic pulse delivery on the combination in a reaction chamber to produce transformed cells, and placing the transformed cells into an organism. The reaction chamber includes a main chamber having a first substantially flat face and which holds a combination of the genetic material, a plurality of cells from the organism and an electronic pulse delivery buffer solution. The reaction chamber also includes a first electrode which has a second substantially flat face which is disposed opposite to and proximate to the first substantially flat face. The first electrode is coupled to receive electronic pulses to perform electronic pulse delivery of the genetic material into at least some of the plurality of cells.
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Milne Andrew
Stone Jacqueline M.
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