Gene therapy using TGF-β

Details Gene therapy using TGF-β Gene therapy using TGF-β

2000-11-08

2008-03-04

Wilson, Michael C. (Department: 1632)

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

C424S093100, C424S093210, C514S04400A

Reexamination Certificate

active

07338655

ABSTRACT:
The subject invention is related to a cell-mediated gene therapy treatment for orthopedic disease using a member belonging to the transforming growth factor-β (TGF-β) superfamily. TGF-β gene therapy as a new treatment method for degenerative arthritis is demonstrated. After transfection of TGF-β cDNA expression vectors into fibroblasts (NIH 3T3-TGF-β1), the cells were injected into rabbit achilles tendon and knee joints with artificially-made cartilage defects. Intratendinous injections were performed to determine the optimal concentration for in vivo expression. Partially defected cartilage model was made to simulate degenerative arthritis of the knee joint. The partial cartilage defect treated with the cell-mediated gene therapy procedure was covered by newly formed hyaline cartilage which indicates that the cells survived and stimulated matrix formation in this area. Completely denuded cartilage areas were covered by fibrous collagen.

REFERENCES:
patent: 4886747 (1989-12-01), Derynck et al.
patent: 5168051 (1992-12-01), Derynck et al.
patent: 5284851 (1994-02-01), Von Deyn et al.
patent: 5482851 (1996-01-01), Derynck et al.
patent: 5700774 (1997-12-01), Hattersley et al.
patent: 5766585 (1998-06-01), Evans et al.
patent: 5801231 (1998-09-01), Derynck et al.
patent: 5842477 (1998-12-01), Naughton
patent: 5846931 (1998-12-01), Hattersley et al.
patent: 5858355 (1999-01-01), Glorioso et al.
patent: 5902741 (1999-05-01), Purchio et al.
patent: 6413511 (2002-07-01), Glorioso et al.
patent: WO96/39196 (1996-12-01), None
patent: WO97/25414 (1997-07-01), None
patent: WO99/11789 (1999-03-01), None
patent: WO99/56785 (1999-11-01), None
Fox, Biological therapies: A novel approach to the treatment of autoimmune disease, 1995, The American Journal of Medicine, vol. 99, pp. 82-88.
Pelletier et al., In vivio suppression of early experimental osteoarthritis by interleukin-1 receptor antagonist using gene therapy, 1997,Arthritis & Rheumatism, vol. 40, pp. 1012-1019.
Crystal, Transfer of genes to humans: Early lessons and obstacles to success, 1995, SCIENCE, vol. 270, pp. 404-410.
Deonarain, Ligan-targeted receptor-mediated vectors for gene delivery, 1998, Exp. Opin. Ther. Patents, vol. 8, pp. 53-69.
Miller et al., Targeted vectors for gene therapy, 1995, FASEB J., vol. 9, pp. 190-199.
Van Beuningen et al., Differential effects of local application of BMP-2 or TGF-beta1 on both articular cartilage composition and osteophyte formation, 1998, Osteoarthritis and Cartilage, vol. 6, pp. 306-317.
Ikeda et al., adenovirus mediated gene delivery to the joints of guinea pigs, 1998, The Journal of Rheumatology, vol. 25, pp. 1666-1673.
Chernajovsky et al., Pathogenic lymphoid cells engineered to express TGF Beta1 ameliorate disease in a collagen-induced arthritis model, 1997, Gene Therapy, vol. 4, pp. 553-559.
MGI website's “Gene Detail” and “Gene Ontology” for TGFb1, no date.
MGI website's “Gene Detail” and “Gene Ontology” for BMP2, no date.
Chernajovsky: Systemic gene therapy for arthritis, Drugs of Today, 35(4-5):361-377, Apr.-May 1999.
Robbins et al.: Gene therapy for rheumatoid arthritis, Springer Seminars in Immunopathology, 20: 197-209, 1998.
Evans and Robbins: Gene therapy of arthritis, Internal Medicine, 38 (3): 233-9, Mar. 1999.
Evans et al.: Blocking cytokines with genes, J Leucocyte Biol., 64:55-61, 1998.
Mason et al.: Expression of bone morphorgenic protein 7 in primary rabbit periosteal cells:˜, Gene Therapy, 1998.
Arai et al.: Adenovirus vector-mediated gene transduction to chondrocytes:˜ J. Rheumatol, 24:1787-1795, 1997.
Otani et al.: Suppression of antigen induced arthritis in Rabbits by ex vivo gene therapy, J Immunol., 156:3558-3562, 1996.
Andrew et al.: Demonstration of TGF-beta-1 mRNA by in situ hybridization in normal fracture healing. Calcif Tissue Int, 52: 74-78, 1993. (Abstract only).
Bourque et al.: Expression of four growth factors during fracture repair. Int J Dev Biol, 37: 573-579, 1993. (Abstract only).
Brand and Schneider: Inactive type II and type I receptors: TGF-beta are dominant inhibitors of TGF-beta-dependent transcription. J Biol Chem, 270: 8274-8284, 1995.
Brittberg et al.: Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. New Engl J Med 331: 889-895, 1994.
Carrington et al.: Accumulation, localization and compartmentation of TGF-beta. during enchondral bone development. J Cell Biology, 107: 1969 1975, 1988.
Centrella et al.: Human platelet-derived transforming growth factor-beta. stimulates parameters of bone growth in fetal rat calvariae. Endocrinology, 119: 2306-2312, 1986.
Cheifetz et al.: Transforming growth factor beta system, a complex pattern of cross-reactive ligands and receptors. Cell, 48: 409-415, 1987.
Chenu et al.: TGF-beta inhibits formation of osteoclast-like cells in long-term human marrow cultures. Proc Natl Acad Sci, 85: 5683-5687, 1988.
Critchlow et al.: The effect of exogenous transforming growth factor. Beta.2 on healing fractures in the rabbit. Bone, 521-527, 1995.
Dallas et al.: Dual role for the latent transforming growth factor beta binding protein in storage of latent TGF-beta˜J Cell Biol, 131: 539-549, 1995.
Dumont et al.: Transforming growth factor receptors on human endometrial cells: identification of the type I and II receptors˜. M Cell Endo, 111: 57-66, 1995.
Frenkel et al.: Chondrocyte transplantation using a collagen bilayer matrix for cartilage repair. J Bone J Surg [Br] 79-B: 831-836, 1997. (Abstract only).
Heine et al.: Role of Transforming Growth Factor-beta in the development of the mouse embryo. Cell Biology, 105: 2861-2876, 1987.
Joyce et al.: Transforming Growth Factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur. J Cell Biology, 110: 2195-2207, 1990.
Lind et al.: Transforming growth factor-beta enhances fracture healing in rabbit tibiae. A Orthop Scand, 64(5): 553-556, 1993. (Abstract only).
Lopez-Casillas et al.: Structure and expression of the membrane proteoglycan component of the TGF-beta receptor system. Cell, 67: 785-795, 1991.
Madri et al.: Phenotypic modulation of endothelial cells by Transforming Growth˜. Depends upon the composition and organization of˜. J Cell Biology, 106: 1375-1384, 1988.
Massague: TGF-Beta Signal Transduction Ann. Rev. Biochem. 67:753-791, 1998.
Matsumoto et al.: Expression and Distribution of Transforming Growth Factor-beta. During Fracture Healing. In vivo, 8: 215-220, 1994. (Abstract only).
Meert et al: Elevated Transforming Growth Factor-beta Concentration˜, The Journal of Trauma, Injury, Infection and Critical Care, vol. 40, No. 6, pp. 901-906, 1996.
Miettinen et al.: TGF-beta Induced Transdifferentiation of Mammary Epithelial Cells˜. Involvement of Type I Receptors. J Cell Biology, 127-6: 2021-2036, 1994.
Ozkaynak et al.: OP-1 cDNA Encodes an Osteogenic Protein in the TGF-beta Family. EMBO J, 9: 2085-2093. (Abstract only).
Sporn and Roberts: Peptide Growth Factors are Multifunctional. Nature (London), 332: 217-219, 1988. (Abstract only).
Wakefield et al.: Latent Transforming Growth Factor-beta from Human Platelets. J Biol Chem, 263: 7646-7654, 1988.
Wrana et al.: Mechanism of Activation of the TGF-beta Receptor. Nature, 370: 341-347, 1994. (Abstract only).
Song et al.: Plasmid DNA Encoding Transforming˜, J. Clin. Investigation, 101: 2615-2621, Jun. 15, 1998.
Sittinger et al.: Joint cartilage regeneration by tissue engineering, Zeitschrift fuer Rheumatologie. 58(3): 130-135, Jun. 1999.
Prud'Homme et al.: Anticytokine Gene Therapy of Autoimmune Diseases. Exp. Opin. Biol. Ther, 1(3):359-373, 2001.
Moller et al.: TGF-beta-1 gentransfer in gelenkknopzellen (TGF beta-1 gene transfer in articular chondrocytes). Orthopade, 29(2): 75-9, Feb. 2000. (English abstract).
Moller and Evans, Genetherapeutische Ansatze in der Arthrosebehandlung (Gene transfer in the treatment of arthritis), Orthopade, 28(1): 76-81, Jan. 1999. (English abstract).

Affiliated with

Also associated with

Rating

Gene therapy using TGF-β does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Gene therapy using TGF-β, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Gene therapy using TGF-β will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3979611