Gene targeting in animal cells using isogenic DNA constructs

Details Gene targeting in animal cells using isogenic DNA constructs Gene targeting in animal cells using isogenic DNA constructs

1997-08-07

1998-08-04

Stanton, Brian R.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4351721, 4353201, 435325, 435326, 800 2, 935 56, 935 70, 935 71, 935 34, 935 53, C12N 506, C12N 1500, C12N 1509, C12N 1511

Patent

active

057892156

ABSTRACT:
The present invention provides novel methods for modifying the genome of an animal cell which typically comprise the steps of: constructing a DNA molecule in which desired sequence modifications are contained in a segment of DNA (a "targeting DNA") that is substantially isogenic with a DNA in the cell genome (a."target DNA"); introducing the targeting DNA construct into the cell (e.g., by microinjection, electroporation, transfection, or calcium phosphate precipitation); and selecting cells in which the desired sequence modifications have been introduced into the genome via homologous recombination.

REFERENCES:
G. Bain et al., Cell, vol. 79, 1994, pp. 885-892.
J. Charron et al., Molecular and Cellular Biology, vol. 10, No. 4, 1990, pp. 1799-1804.
C. Deng and M.R. Capecchi, Molecular and Cellular Biology, vol. 12, No. 8, 1992, pp. 3365-3371.
C. Deng et al., Molecular and Cellular Biology, vol. 13, No. 4, 1993, pp. 2134-2140.
B. Koller et al., Proc. Natl. Acad. Sci. USA, vol. 88, 1991, pp. 10730-10734.
A.H. Schinkel et al., Cell, vol. 77, 1994, pp. 491-502.
J. van Deursen and B. Wieringa, Nucleic Acids Research, vol. 20, No. 15, 1992, pp. 3815-3820.
van der Lugt et al., Genes & Development, vol. 8, 1984, pp. 757-769.
H. Zheng and E. Wilson, Naturevol. 344, 1990, pp. 170-173.
Adair et al., Proc. Natl. Acad. Sci. USA 86:4574-4578 (1989).
Bollag et al., Annual Review of Genetics 23:199-225 (1989).
Jasin and Berg, Genes & Development 2:1353-1363 (1988).
Joyner et al., Nature 338:153-156 (1989).
Mansour et al., Nature 336:348-352 (1988).
Sedivy and Sharp, Proc. Natl. Acad. Sci. USA 86:227-231 (1989).
Shen and Huang, Genetics 112:441-457 (1986).
Song et al., Proc. Natl. Acad. Sci. USA 84:6820-6824 (1987).
Soriano et al., Cell 64, 693-702 (1991).
Swebilius Singer et al., Cell 31:25-33 (1981).
Waldman and Liskay, Molecular and Cellular Biology 8:5350-5357 (1988).
Watt et al., Proc. Natl. Acad. Sci. USA 82:4768-4772 (1985).
Zijlstra et al., Nature 342:435-438 (1989).
Zimmer et al., Nature 338:150-153 (1989).
Lowy et al. 1980 Cell 22:817-823.
Charron et al. (1990) Molecular: Cellular Biology 10(4): 1799-1805.
Fell et al. (1989) PNAS, USA 86: 8507-8511.
Cruz et al. (1990) Nature 348:171-173.
Schwartzberg et al. (1989). Science 246:799-803.
Baker et al., (1988). Molecular and Cellular Biology 8(10): 4041-4047.
ten Asbroek et al. 1990. Nature 348:174-175.
Rubinitz et al. 1984. Mol. Cell. Biol. 4(11): 2253-2258.
Van der Lugt, N., et al., Gene, 105:263-267 (1991).
Te Riele, H., et al., Nature 348:649-651 (1990).
Hooper, M., et al., Nature 326:292-295 (1987).
Yenofsky, R., et al., Proc. Natl. Acad. Sci. (U.S.A.) 87:3435-3439 (1990).
Waldman, A.S. and Liskay, R.M., Proc. Natl. Acad. Sci. (U.S.A.) 4:5340-5344 (1987).
Camerini-Otero & Kucherland, The New Biologist 4:337-341 (1990).
Doetschman et al., Proc. Natl. Acad. Sci. (U.S.A.) 85:8583-8587 (1988).
Doetschman et al., Nature 330:576-578 (1988).
Thomas et al., Cell 44:419-428 (1986).
Koller et al., Proc. Natl. Acad. Sci. (U.S.A.) 86:8927-8931 (1989).
Smithies et al., Nature 317:230-234 (1985).
Thomas & Capecchi, Cell 51:503-512 (1987).

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