Gene synthesis method

Details Gene synthesis method Gene synthesis method

1996-12-20

2000-08-29

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 536 253, C12Q 168, C12P 1934

Patent

active

061106682

ABSTRACT:
A method of gene synthesis is disclosed. The gene synthesis method permits the codons of [m] natural gene to be changed to allow preferential transcription and translation of the synthetic gene in transgenic organisms. The method utilizes a combination of enzymatic and chemical synthesis of DNA and significantly reduces the cost, time and number of steps required for construction of synthetic genes.

REFERENCES:
patent: 4652639 (1987-03-01), Stabinsky
patent: 4888286 (1989-12-01), Crea
patent: 5158877 (1992-10-01), Edwards et al.
patent: 5367066 (1994-11-01), Urdea et al.
patent: 5380831 (1995-01-01), Adang et al.
patent: 5503995 (1996-04-01), Khudyakov et al.
patent: 5506137 (1996-04-01), Mathur et al.
patent: 5547843 (1996-08-01), Studier et al.
patent: 5593840 (1997-01-01), Bhatnagar et al.
Wiedmann et al., Appl. Environ. Microbiol. 59(8), 2743-2745, 1993.
Weisberg, Edward P. eet al., "Simultaneous Mutagenesis of Multiple Sites: plication of the Ligase Chain Reaction Using PCR Products Instead of Oligonucleotides", BioFeedback, vol. 15, No. 1, (1993)pp. 68-75.
Kozak, Marilyn, "Downstream Secondary Structure Facilitates Recognition of Initiator Codons by Eukaryotic Ribosomes", Proc. Natl. Acad. Sci USA, Nov. 1990, vol. 87, pp. 8301-8305.

Affiliated with

Also associated with

Rating

Gene synthesis method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Gene synthesis method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Gene synthesis method will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1248294