Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1981-12-09
1985-04-02
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 68, 435 91, 435253, 435317, 435874, 435875, 435 23, 435 29, 435 56, 435 72, 435 80, C12N 1500, C12N 120, C12N 100, C12P 2100, C12P 1934, C12R 138, C12R 1385
Patent
active
045088233
ABSTRACT:
An improved gene splicing and recombinant plasmid transformation method is described. The method includes mechanical fragmenting of chromosomal DNA followed by conventional digestion with a restriction enzyme and gene splicing into a vector to provide recombinant plasmids in a bank of at least about 100 different plasmids. The plasmids in the bank are provided for transformation into a suitable host, particularly a plasmid free bacterium of the same species from which the chromosomal DNA or the vector is derived. The method provides high transformation frequencies because of the presence of multiple "super coiled" or closed coiled recombinant plasmids in the bank. The method also allows for the direct selection of many different phenotypic traits in a pool of the transformed hosts. The selected hosts are useful for the production of various gene products.
REFERENCES:
patent: 3813316 (1974-05-01), Chakrabarty
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4278765 (1981-07-01), Debabov et al.
patent: 4374200 (1983-02-01), Olsen
patent: 4376164 (1983-03-01), Olsen
patent: 4418194 (1983-11-01), Olsen
Freifekder: Molecular Biology, Science Books International, 1983, pp. 107-108.
Wensink et al: Cell 3, 315, (1974).
Helling et al: in Genetic Engineering, Chakrabarty (ed.), CRC Press, 1978, pp. 1-30.
Macrina, F. D. Kepecko, K. Jones, D. Ayers and S. McCowen Plasmid 14:7-420, (1978).
Hansen, J. and R. Olsen, J. Bacteriol. 135:227-238, (1978).
Royle, Matsumoto and Holloway, J. Bacteriol. 145:145-155, (1981).
Bolivar, R., et al. Gene 2:95-113, (1977).
Bagdasarian et al, in: Plasmids of Medical, Environmental and Commercial Importance, Timmis et al. (Ed.), Elsevierl North-Holland, 1979, pp. 411-422.
Wood et al., J. Bacteriol. 145: 1448-1451, (1981).
Ditta et al: Proc. Natl. Acad. Sci. USA 77:7347-7351, (1980).
Guerry, LeBlanc and Falkow, J. Bacteriol. 116:1064-1066, (1973).
Mercer and Loutit, J. Bacteriol. 140:37-42, (1979).
Carbon, J. et al, Recombinant Molecules: Impact on Science and Society, Raven Press, New York, (1977), pp. 355-378.
Clarke, L., and J. Carbon, Proc. Nat. Acad. Sci. USA 72: 4361-4365, (1975).
Martinell James
McLeod Ian C.
Microlife Technics, Inc.
Wiseman Thomas G.
LandOfFree
Gene splicing method and products produced therefrom does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Gene splicing method and products produced therefrom, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Gene splicing method and products produced therefrom will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1091521