Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1994-07-19
1996-12-31
Jones, W. Gary
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 6, 536 235, C12Q 168, C07H 2102
Patent
active
055895794
ABSTRACT:
The present invention discloses an isolated and purified nucleic acid sequence and corresponding amino acid sequence to a novel protein specific for human lung cancer cells. This gene is expressed at a much higher level in these cells than in normal lung, other normal tissues and other tumor cell lines tested. Also disclosed are two additional recombinant forms of this gene and protein, in the first case a membrane spanning region is removed and in the second case an amino acid is changed by in vitro mutagenesis. Also disclosed are nucleic acid probes for the detection of lung cancer cells from tissue biopsy and body fluids such as serum, sputum and bronchial washings. A method for expressing the antigen in a host cell and its subsequent use as an immunogen in antibody production for test applications is described. An ELISA test to measure shed antigen present in patient samples as well as an enzyme test to measure activity in specimens also is described.
REFERENCES:
patent: 4816402 (1989-03-01), Rosen et al.
patent: 4990454 (1991-02-01), Yachi et al.
patent: 5134075 (1992-07-01), Hellstrom et al.
patent: 5185432 (1993-02-01), Hellstrom et al.
patent: 5200508 (1993-04-01), Nilaver et al.
Mori, et al., "The significance of carbonic anyhdrase expression in human colorectal cancer," Gastroenterology 105:820-826 (1993). Abstract only.
Skonier, et al., "cDNA cloning and sequence analysis of .beta.ig-h3, a novel gene induced in a human adenocarcinoma cell line after treatment with transforming growth factor-.beta.," DNA Cell Biology 11:511-522 (1992).
Mason and Housley, J. Biol. Chem. 268(24): pp. 21501-21504 (1993).
Anastasi, et al., "Direct correlation of cytogenetic findings with cell morphology using in situ hybridization: an analysis of suspicious cells in bone morrow specimens of two patients completing therapy for acute lymphoblastic leukemia," Blood 77:2456-2462 (1991).
Altschul, et al., "Basic local alignment search tool," J. Molecular Biol 215:403-410 (1990).
Birrer, et al., "Application of molecular genetics to the early diagnosis and screening of lung cancer," Cancer Research (supplement) 52:2658s-2664s (1992).
Boring, et al., "Cancer statistics, 1993," CA Cancer J Clin 43:7-26 (1993).
Chirgwin, et al., "Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease," Biochem 18:5294-5299 (1979).
Diez, et al., "Prognostic significance of serum CA 125 antigen assay in patients with non-small cell lung cancer," Cancer 73:1368-1376 (1994).
Flehinger, et al., "The effect of surgical treatment on survival from lung cancer: implications for screening," Chest 101:1013-1018 (1992).
Gray, J W and Pinkel, D, "Molecular cytogenetics in human cancer diagnosis," Cancer (supplement) 69:1536-1542 (1992).
Hasegawa, et al., "Nonspecific crossreacting antigen (NCA) is a major member of the carcinoembryonic antigen (CEA)-related gene family expressed in lung cancer," British J Cancer 67:58-65 (1993).
von Heijne, G., "A new method for predicting signal sequence cleavage sites," Nucleic Acid Research 14:4683-4690 (1986).
Kern, et al., "p185.sup.neu Expression in human lung adenocarcinomas predicts shortened survival," Cancer Research 50:5184-5191 (1990).
Kim, et al., "Interphase cytogenetics in paraffin sections of lung tumors by non-isotopic in situ hybridization," Am J Path 142:307-317 (1993).
Kunkel, T. A., "Rapid and efficient site-specific mutagenesis without phenotypic selection," Proc Natl Acad Sci USA 82:488-492 (1985).
Margolis, et al., "Serum tumor markers in non-small cell lung cancer," Cancer 73:605-609 (1994).
Melamed, et al., "Screening for early lung cancer: results of the Memorial Sloan-Kettering study in New York," Chest 86:44-53 (1984).
Mitsudomi, et al., "p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features," Oncogene 7:171-180 (1992).
Mulshine, et al., "Section 22.5 Applications of monoclonal antibodies in the treatment of solid tumors," Biologic Therapy of Cancer, V T DeVita, S. Hellman, S A Rosenberg (ed), J B Lippencott Co., New York, pp. 563-588 (1991).
Parkin, et al., "Estimates of the worldwide incidence of eighteen major cancers in 1985," Int J Cancer 54:594-606 (1993).
Radosevich, et al., "Monoclonal antibody assays for lung cancer," Cancer Diagnosis In Vitro Using Monoclonal Antibodies, H. Kupchik (ed.), Marcel Dekker, Inc., New York, pp. 101-121 (1988).
Schepart, B S and Margolis, M L, "Monoclonal antibody-mediated detection of lung cancer antigens in serum," Am Rev Respir Dis 138:1434-1438 (1988).
Scott, et al., "Early lung cancer detection using monoclonal antibodies," Lung Cancer, J A Roth, J D Cox, and W K Hong (eds), Blackwell Scientific Publications, Boston, pp. 310-323 (1993).
Smith, D B and Johnson, K S, "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase," Gene 67:31-40 (1988).
Souhami, et al., "Antigens of lung cancer: results of the Second International Workshop on Lung Cancer Antigens," J Natl Cancer Institute 83:609-612 (1991).
Strnad, et al., "Molecular cloning and characterization of a human adenocarcinoma/epithelial cell surface antigen complementary DNA," Cancer Research 49:314-317 (1989).
Tockma, et al., "Sensitive and specific monoclonal antibody recognition of human lung cancer antigen on preserved sputum cells: a new approach to early lung cancer detection," J Clin Oncology 6:1685-1693 (1988).
Anon, "Early lung cancer detection: summary and conclusions," Am Rev Respir Dis 130:565-570 (1984).
"The World Health Organization histological typing of lung tumours (second edition)," Am J Clin Path 77:123-136 (1982).
Bollon Arthur P.
Torczynski Richard M.
Cytoclonal Pharmaceutics, Inc.
Jones W. Gary
Whisenant Ethan
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