Gene regulation cassettes, expression vectors containing the sam

Details Gene regulation cassettes, expression vectors containing the sam Gene regulation cassettes, expression vectors containing the sam

1992-10-19

1995-06-27

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 6952, 4352523, 43525233, 4353201, 536 241, 935 29, 935 41, 935 47, 935 73, C12N 1567, C12N 1570, C12N 1571, C12N 1573

Patent

active

054279240

ABSTRACT:
The present invention relates to gene regulation cassettes useful for (1) detecting the specific mRNA sequences which best enhance the translation efficiency of mRNA into protein when a specific foreign gene is adjacent thereto and (2) expressing foreign genes in procaryotic cells; expression vectors containing the gene regulation cassettes adjacent to a foreign gene such that the expression of the foreign gene is under the control of the gene regulation cassettes; and microorganisms transformed with the expression vectors. In the former gene regulation cassette, a restriction site encompasses or is adjacent to 2 base pairs 3' of the start of mRNA transcription of the lambda P.sub.R operator/promoter. This gene regulation cassette is useful for inserting and selecting sequences that generate a favorable rate of translation efficiency of the foreign gene. In the latter gene regulation cassette, a lambda P.sub.R operator/promoter is fused to a modified ribosome binding site. This gene regulation cassette is useful for obtaining a favorable rate of translation efficiency of, inter alia, the IL-1.beta. gene and a truncated form of the M-CSF gene.

REFERENCES:
Zabeau et al; EMBO J. 1: 1217 (1982).
Stewart et al; Plasmid 15: 182 (1986).
Bressan et al; Nucleic Acids Res. 15: 10056 (1987).

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