Gene participating in the mechanism of secretion of esterase

Details Gene participating in the mechanism of secretion of esterase Gene participating in the mechanism of secretion of esterase

1998-01-09

1999-07-13

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

536 231, 536 237, 4352523, 4353201, 435196, 435880, 530350, C12P 2106, C12N 120, C12N 916, C07H 2104

Patent

active

059225688

ABSTRACT:
An isolated gene encoding a polypeptide which is required for secretion of esterase originated from a microorganism of the genus Serratia, a recombinant plasmid comprising a plasmid prepared by inserting the isolated gene into a vector plasmid, a microorganism transformed with the recombinant plasmid, and a method for the production of an esterase which comprises cultivating the transformant microorganism as set forth above in a medium and collecting the produced esterase outside and inside the cells. The transformed microorganism have remarkably excellent capability of extracellular secretion of esterase.

REFERENCES:
patent: 5846811 (1998-12-01), Shibatani et al.
Akatsuka, et al., "The Three Genes lipB, lipC, and lipD Involved in the Extracellular Secretion of Serratia marcescens Lipase Which Lacks an N-Terminal Signal Peptide," Journal of Bacteriology, vol. 177, No. 22 (Nov. 1995) pp. 6381-6389.

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