Gene mapping by hybridization to free chromatin

Details Gene mapping by hybridization to free chromatin Gene mapping by hybridization to free chromatin

1993-09-16

1995-11-28

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 77, 935 78, C12Q 168

Patent

active

054707099

ABSTRACT:
A method is provided for detecting, ordering and mapping genes or DNA sequences in the genome of eukaryotic cells. The method comprises the steps of releasing free chromatin from the nuclei of the cells and contacting the released free chromatin with at least one detectable probe capable of hybridizing to the genes or DNA sequences to be detected, thereby rendering the genes or DNA sequences detectable.

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Heng et al., High-Resolution mapping of mammalianenes by in situ hybridization to free chromatin (Oct. 1992) PNAS (89) pp. 9509-9513.
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