Gene involved in pyrimidine biosynthesis in plants

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se

Reexamination Certificate

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Details

C435S006120, C435S069100, C435S468000, C435S419000, C435S252300, C435S320100, C435S183000, C530S370000, C536S023600, C800S278000

Reexamination Certificate

active

06906242

ABSTRACT:
This invention relates to an isolated nucleic acid fragment encoding an OMP decarboxylase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the OMP decarboxylase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the OMP decarboxylase in a transformed host cell.

REFERENCES:
Philip Reyes et al., Journ. of Biol. Chem., vol. 250 (13):5097-5108, Jul. 10, 1975, Studies on a Pyrimidine Phosphoribosyltransferase from Murine Leukemia P1534J.
Thomas W. Traut et al., Biochemistry, vol. 19:6062-6068, 1980, Dependence of the Aggregation and Conformation States of Uridine 5′-Phosphate Synthase on Pyrimidine Nucleotides. Evidence for a Regulatory Site.
Keith Shostak et al., Biochemistry, vol. 31:12155-12161, 1992, Orotidylate Decarboxylase: Insights into the Catalytic Mechanism from Substrate Specificity Studies.
National Center for Biotechnology Information General Identifier No. 2499945, May 30, 2000, Nasr, F. et al., Heterospecific Cloning ofArabidopsis thalianacDNAs by Direct Complementation of Pyrimidine Auxotrophic Mutants ofSaccharomyces cerevisiae.I. Cloning Sequence Analysis of two cDNAs Catalysing the second, fifth and sixth steps of the de novo Pyrimidine Biosynthesis Pathway.
Fahd Nasr et al., Mol. Gen. Genet. vol. 244:23-32, 1994, Heterospecific Cloning ofArabidopsis thalianacDNAs by Direct Complementation of Pyrimidine Auxotrophic Mutants ofSaccharomyces cerevisiae.I. Cloning and Sequence Analysis of two cDNAs Catalysing the second, fifth and sixth steps of the de novo Pyrimidine Biosynthesis Pathway.
National Center for Biotechnology Information General Identifier No. 2499946, May 30, 2000, Maier, T. et al., Nucleotide Sequence of a cDNA Encoding UMP Synthase fromNicotiana tabacum.
Ulrich Strych et al., Current Microbiology, vol. 29:353-359, 1994, Orotidine-5′-Monophosphate Decarboxylase fromPseudomonas aeruginosaPA01: Cloning, Overexpression, and Enzyme Characterization.
Kristen K. Seymour et al., Biochemistry, vol. 33:5268-5274, 1994, Cytotoxic Effects of Inhibitors of de Novo Pyrimidine Biosynthesis uponPlasmodium falciparum.

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