Gene integration into chromosomes of lactobacillus delbrueckii s

Details Gene integration into chromosomes of lactobacillus delbrueckii s Gene integration into chromosomes of lactobacillus delbrueckii s

1996-12-04

1998-05-05

Ketter, James

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352523, 4352529, 4353201, C12N 1563, C12N 121

Patent

active

057473102

ABSTRACT:
A gene to be integrated is bound to pAM.beta.1 plasmid to construct an integration plasmid, which is conjugally transferred into chromosomal DNA of a strain of Lactobacillus delbrueckii species. Integration of only the objective gene occurs through homologous recombination events (double crossing-over). According to this method, an arbitrary gene can be integrated into chromosomal DNA of a strain of Lactobacillus delbrueckii species and the DNA sequence derived from the vector, pAM.beta.1, can be completely removed. Therefore, the obtained gene integrant is widely applicable to the production of foods, medicines, feeds and the like.

REFERENCES:
Schwarzer and Puhler Biotechnology 9 84-87 (1991) Manipulation of orynebacterium glutamicum by Gene Disruption . . .
Sasaki, et al., FEMS Microbiol. Rev. 87.17 (Abstr).
Ragout, et al. Biochimie 71 639-644 (1989) Presence of an L(+)-Lactate Dehydrogenase in Cells of Lactobicillus delbureckii. . .
Bernard, et al. FEBS Letters 290 61-64 (1991) Cloning of the D-Lactate Dehydrogenase Gene From Lactobacillus delbrueckii . . .
Clewell et al.; "Characterization of 3 Plasmid DNA Molecules in a Strain of Steptococcusfaecalis Identification of a Plasmid Determining Erythromycin Resistance"; Journal of Bacteriology; vol. 117, No. 1, 1974, pp. 283-289.

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