Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters plant part growth
Reexamination Certificate
1998-09-18
2001-10-02
Benzion, Gary (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters plant part growth
C435S468000, C435S320100, C536S023600, C800S298000, C800S317000, C800S323100
Reexamination Certificate
active
06297429
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a gene encoding a transcription factor capable of altering characters of a plant and its use. More particularly, the present invention relates to the PetSPL3 gene which is a novel gene derived from
Petunia hybrida,
genes related thereto, and the use thereof.
2. Description of the Related Art
In order to clarify regulatory mechanisms controlling the characters of a plant, for example, morphogenesis of a flower, molecular biological and molecular geneticical studies have been conducted using
Arabidopsis thaliana, Antirrhinum majus,
and
Petunia hybrida.
In particular,
Petunia hybrida
is preferably used as a subject of studies for the following reasons: high value as a horticultural plant: the presence of various species; ease of transformation; ease to observe due to its large flower, and accumulation of genetical findings (H. Takatsuji, “Molecular mechanism for determining a shape of a plant”, Cell Technology, Plant Cell Technology Series (SHUJUNSHA), pp. 96-106).
Genes which cause mutation have been isolated from mutants in which floral organs of the above-mentioned plant is altered. As a result, it is becoming clear that transcription factors play important roles in differentiation and morphogenesis of a flower. For example, SUPERMAN of
Arabidopsis thaliana
is a transcription factor having a zinc finger motif as a DNA binding domain. It is known that, in SUPERMAN mutant, the number of stamens are remarkably increased, and pistils are defective (THE IDEN, April, 1997 (Vol. 51, No. 4), pp. 34-38).
For understanding the mechanism for the control of characters of a plant, it is important to identify a novel transcription factor which is involved in the control. A gene for a transcription factor which controls morphogenesis of a flower may be introduced into a plant by using gene engineering procedure. It is possible to obtain a plant, using gene introduction, having a flower with novel characters which has not been obtained or is not likely to be obtained by a conventional breeding. It is considered that a plant with such novel characters is horticulturally valuable.
SUMMARY OF THE INVENTION
A gene of the present invention has DNA which is selected from a) or b): a) DNA having a nucleotide sequence from the 90th position to the 728th position of a nucleotide sequence represented in SEQ ID NO: 1 of Sequence Listing; or b) DNA which hybridizes to DNA of a) under stringent conditions, and encodes a transcription factor capable of altering characters of a plant.
A gene of the present invention encodes a transcription factor which is selected from i) or ii):
i) a transcription factor having an amino acid sequence from the 1st position to the 213rd position of an amino acid sequence represented in SEQ ID NO: 2; or ii) a transcription factor having an amino acid sequence in which one or more amino acids of i) are subjected to deletion, substitution, or addition, and being capable of altering characters of a plant.
In one embodiment of the present invention, the characters of a plant include one selected from the group consisting of morphology of a flower, color of a flower, a size of a plant, and the number of branches.
A method for producing a transgenic plant of the present invention includes the steps of: introducing a plant cell with the above-mentioned gene: and regenerating a plant body from the plant cell with the introduced-gene.
In one embodiment of the present invention, the plant belongs to dicotyledon.
In another embodiment of the present invention, the plant belongs to Solanaceae.
In another embodiment of the present invention, the plant belongs to Petunia.
In another embodiment of the present invention, the gene is incorporated into a plant expression vector.
A transgenic plant of the present invention is produced by the above-mentioned method.
Thus, the invention described herein makes possible the advantages of (1) providing a gene encoding a transcription factor capable of altering characters of a plant; (2) providing a method for producing a plant with its character altered by introduction of the gene; and (3) providing a transgenic plant.
These and other advantages of the present invention will become apparent to those skilled in the art upon reading and understanding the following detailed description with reference to the accompanying figures.
REFERENCES:
patent: WO 90/12084 (1990-10-01), None
van der Krol et al, Nature, vol. 333, pp. 866-869, 1988.*
Kubo, K. et al., “CyS2/His2 zinc-finger protein family of petunia: evolution and general mechanism of target-sequence recognition,”Nuc. Acids Res., vol. 26, No. 2, pp. 608-615 (1998).
Sakai, H. et al. “Role of SUPERMAN in maintaining Arabidopsis floral whorl boundaries.”Nature378:199-203 (1995).
Tangue, B.W. and Goodman, H.M. “Characterization of a family of Arabidopsis zinc finger protein cDNAs.”Plant mol. Biol.28:267-279 (1995). (Abstract and EMBL sequence information enclosed.)
Takatsuji Hiroshi. “Transcription Factors Controlling Floral Organ Development.”Cell Technology.Plant Technology Series. Shujunsha, Japan, pp. 96-106.
Takatsuji, Hiroshi.The IDEN, vol. 51, No. 4, pp. 34-38 (1997).
Nakagawa Hitoshi
Takatsuji Hiroshi
Benzion Gary
Director General of National Institute of Agrobiological Resourc
Mehta Ashwin D.
Townsend and Townsend / and Crew LLP
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