Gene expression vector using the gene expression regulating regi

Details Gene expression vector using the gene expression regulating regi Gene expression vector using the gene expression regulating regi

1995-04-07

1998-06-30

Robinson, Douglas W.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

4353201, 435421, 536241, C12N 1582, C12N 1563, C07H 2104, A01H 500

Patent

active

057736880

ABSTRACT:
cDNA derived from the ARF gene that is actively expressed in barley seeds, roots and callus, but is highly suppressed or almost not expressed at all in leaves, was cloned. Next, the upstream region of the ARF gene was cloned, its promoter activity was verified, and a gene expression vector using the expression region of this gene was produced.

REFERENCES:
Kim et al. A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity. Plant Molecular Biology 24:105-117, 1994.
Serventi, et al. Characterization of the gene for ADP-ribosylation factor (ARF) 2, a developmentally regulated, selectively expressed member of the ARF family of .about.20-kDa guanine nucleotide-binding proteins. The Journal of Biological Chemistry. 266(7):4, 1993.
Randy S. Haun et al., "Characterization of the Human ADP-Ribosylation Factor 3 Promoter", The Journal of Biological Chemistry, vol. 268, No. 1, pp. 8793-8800, Apr. 25, 1993.
Febs Letters, vol. 316, No. 2, pp. 133-136, 1993, F. Regad, et al., "CDNA Cloning and Expression of an Arabidopsis GTP-Binding Protein of the ARF Family".
Journal of Biological Chemistry, vol. 268, No. 7, pp. 4863-4872, Mar. 5, 1993, I.M. Serventi, et al., "Characterization of the Gene for ADP-Ribosylation Factor (ARF) 2, A Developmentally Regulated Selectively Expressed Member of the ARF Family of .about.20-KDA Guanine Nucleotide-Binding Proteins".
The EMBO Journal, vol. 9, No. 6, pp. 1677-1684, 1990, Philip N. Benfey, et al., "Tissue-Specific Expression from CaMV 35s Enhancer Subdomains in Early Stages of Plant Development".
The Plant Cell, vol. 2, pp. 163-171, Feb. 1990, David McElroy, et al., "Isolation of an Efficient Actin Promoter for Use in Rice Transformation".

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