Gene expression using T cell factor responsive element

Details Gene expression using T cell factor responsive element Gene expression using T cell factor responsive element

2007-02-20

2007-02-20

Priebe, Scott D. (Department: 1633)

Chemistry: molecular biology and microbiology

Vector, per se

C435S325000, C435S455000, C435S456000, C424S093200, C514S04400A

Reexamination Certificate

active

10607479

ABSTRACT:
Disclosed are DNA elements and constructs useful for obtaining tumor-selective gene expression in tumors having a mutated β-catenin/APC pathway. In particular, the use of these constructs to express genes encoding therapeutic proteins in colorectal cancer cells is described. The constructs comprise multiple repeats of a TCF-binding element operably linked to a promoter. By means of such a construct, tumor cell-specific expression of a prodrug-converting enzyme such as nitroreductase may be achieved. Coupled with systemic administration of a suitable prodrug, such as CB1954, selective killing of such tumor cells can be demonstrated.

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