Gene expression system comprising the promoter region of the alp

Details Gene expression system comprising the promoter region of the alp Gene expression system comprising the promoter region of the alp

1994-11-22

1998-01-27

Campell, Bruce R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 697, 435 698, 435 701, 4351723, 435410, 435420, 536 231, 536 236, 536 241, 935 35, 935 60, 935 67, C12P 2106, C12P 2102, C12N 500, C12N 1500

Patent

active

057121125

ABSTRACT:
The present invention is directed to a method for producing a gene product by expressing a gene encoding the gene product in angiosperm host cells, comprising the steps of constructing a vector expressible in angiosperm host cells, the vector comprising a promoter region derived from an .alpha.-amylase gene of a plant, and a gene encoding a desired gene product; transforming a compatible angiosperm host cell with the vector; cultivating the resultant transformant host cell; subjecting the cultivated transformant host cell to a sugar-depleted or sugar-free condition to promote the expression of the gene under the control of the promoter region; and recovering the expressed gene product. The present invention also is directed to a method for the production of a transgenic plant of rice crop comprising the steps of infecting an immature embryo of rice crop with the genus Agrobacterium for transformation; co-culturing the infected embryo with a dicot suspension culture during the step of transformation; allowing the transformed embryo to grow into a callus in a selective medium comprising a sufficient amount of a plant growth hormone for the growth of rice crop; and allowing the cultured callus to regenerate root and shoot in a regeneration medium comprising a pre-determined amount of nutrients for the growth of rice crop. The invention is further directed to a transformed rice plant made by methods of this invention.

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