Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1992-11-04
1995-10-24
Fox, David T.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 701, 435 697, 435 698, 4351723, 4352404, 43524046, 536 231, 536 241, 536 236, 935 35, 935 60, 935 67, C12P 2106, C12P 2102, C12N 1500, C12N 500
Patent
active
054609526
ABSTRACT:
Disclosed is a method for producing a gene product by expressing a gene encoding said gene product in plant host cells, comprising the steps of: constructing a vector expressible in plant host cells, the vector comprising a promoter region derived from an .alpha.-amylase gene of a plant, and a gene encoding a desired gene product; transforming a compatible plant host cell with the vector; cultivating the resultant transformant host cell; subjecting the cultivated transformant host cell to a sugar-depleted or sugar-free condition to promote the expression of the gene under the control of the promoter region; and recovering the expressed gene product. Also disclosed is a method for producing a gene product method which comprises the steps of: constructing a vector expressible in plant host cells, the vector comprising a promoter region derived from an .alpha.-amylase gene of a plant, and a gene encoding a desired gene product, the promoter region including the promoter and a DNA sequence encoding the signal peptide; transforming a compatible plant host cell with the vector; cultivating the resultant transformant host cell in a suitable culture medium; and directly recovering the expressed gene product from the medium.
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Liu Li-Fei
Yu Su-May
Campell Bruce
Fox David T.
National Science Counsil of R.O.C.
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