Gene expression system based on regulatory elements of baterioph

Details Gene expression system based on regulatory elements of baterioph Gene expression system based on regulatory elements of baterioph

1993-03-08

1994-09-13

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Vector, per se

43525233, 536 237, 536 241, C12N 1570, C12N 121, C12N 1511, C12N 1531

Patent

active

053468309

ABSTRACT:
An E. coli heterologous gene expression system comprising a multicopy expression vector comprising an F promoter from bacteriophage P2; and at least one copy of a DNA sequence comprising a delta gene from satellite P4 operably linked to a regulatable promoter on a different replicon is disclosed.

REFERENCES:
Suzuki et al. (1986), An Introduction to Genetic Analysis, Third Edition, (W. H. Freeman & Co., New York) pp. 385-392.
Slonczewski et al. (Jul. 1987), J. Bacteriol., vol. 169(7), pp. 3001-3006.
Souza, L., et al., "A Transactivation Mutant of Satellite Phage P4," Virology 81:81-90 (1977).
Studier, F. W. and B. A. Moffatt, "Use of Bacteriophage T7 RNA Polymerase to Direct Selective High-Level Expression of Cloned Genes," J. Mol. Biol. 189:113-130 (1986).
Grambow, N. J., et al., "Deletion Analysis of a Bacteriophage P2 Late Promoter," Gene 95:9-15 (1990).
Christie, G. E. and R. Calendar, "Interactions Between Satellite Bacteriophage P4 and Its Helpers," Annu. Rev. Genet. 24:465-490 (1990).

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