Gene expression system

Details Gene expression system Gene expression system

2008-05-13

2008-05-13

Ketter, James S (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S320100, C435S235100, C435S091410, C435S325000

Reexamination Certificate

active

07371542

ABSTRACT:
The present invention relates to nucleic acid molecules and in particular to vectors, comprising at least one gene of interest, at least one scaffold/matrix attached region (S/MAR), at least one origin of replication, and at least one replication initiation factor, cells comprising these, processes for their propagation, and their use, in particular for the high level expression of proteins which can be used as medicaments.

REFERENCES:
patent: 4686186 (1987-08-01), Sugden
patent: 5580859 (1996-12-01), Felgner et al.
patent: 5705163 (1998-01-01), Pastan et al.
patent: 6194212 (2001-02-01), Agarwal et al.
patent: 6410314 (2002-06-01), Baiker et al.
patent: 0 716 148 (1994-09-01), None
patent: 0 716 148 (1994-09-01), None
patent: WO 95/27020 (1995-03-01), None
patent: WO 96/17072 (1996-06-01), None
patent: WO 97/46687 (1997-12-01), None
patent: WO 98/13511 (1998-04-01), None
patent: WO 99/50432 (1999-03-01), None
patent: WO 2004/053137 (2004-06-01), None
patent: WO 2004/053137 (2004-06-01), None
Agapov, E.V., et al., “Noncytopathic Sindbis virus RNA vectors for heterologous gene expression,”Proc. Natl. Acad. Sci. USA 95:12989-12994, The National Academy of Sciences (1998).
Aiyar, A., et al., “The plasmid replicon of EBV consists of multiplecis-acting elements that facilitate DNA synthesis by the cell and a viral maintenance element,”EMBO J. 17:6394-6403, Oxford University Press (1998).
Altman, A.L., and Fanning, E., “Defined Sequence Modules and an Architectural Element Cooperate To Promote Initiation at an Ectopic Mammalian Chromosomal Replication Origin,”Mol. Cell. Biol. 24:4138-4150, American Society for Microbiology (May 2004).
Berglund, P., et al., “Alphaviruses as vectors for gene delivery,”Trends Biotechnol. 14:130-134, Elsevier Science Ltd. (1996).
Blasey, H.D., et al., Mammalian Transient Gene Expression-Potential, Practical Application and Perspectives in:Animal Cell Technology:Products from Cells, Cells as Products, Bernard, A., et al., eds., Kluwer Academic Publishers, Norwell, MA, pp. 331-337 (1999).
Bode, J. et al., “The Hitchhiking Principle: Optimizing episomal vectors for the use in gene therapy and biotechnology,”Gene Ther. Mol. Biol. 6:33-46, Gene Therapy Press (2001).
Bohl, D., et al., “Long-term control of erythropoietin secretion by doxycycline in mice transplanted with engineered primary myoblasts,”Nat. Med. 3:299, Nature Publishing Company (1997).
Boorsma, M., et al., “A temperature-regulated replicon-based DNA expression system,”Nature Biotech. 18:429-432, Nature America Inc. (2000).
Borrsma, M., et al., “Alphavirus cDNA-Based Expression Vectors: Effects of RNA Transcription and Nuclear Export,”Biotechnol. Bioeng.81:553-562, Wiley Periodicals Inc. (Mar. 2003).
Bredenbeek, P.J., et al., “Sindbis Virus Expression Vectors: Packaging of RNA Replicons by Using Defective Helper RNAs,”J. Virol. 67:6439-6446, American Society for Microbiology (1993).
Brown, J.W., “The Ribonuclease P Database,”Nucleic Acids Res. 26:351-352, Oxford University Press (1998).
Burge, B.W. and Pfefferkorn, E.R., “Complementation Between Temperature-sensitive Mutants of Sindbis Virus,”Virol. 30:214-223, Academic Press (1966).
Burge, B.W. and Pfefferkorn, E.R.,“Isolation and Characterization of Conditional-lethal Mutants of Sindbis Virus,”Virol. 30:204-213, Academic Press (1966).
Ceccarelli, D. F. and Frappier, L., “Functional Analyses of the EBNA1 Origin DNA Binding Protein of Epstein-Barr Virus,”J. Virol. 74:4939-48, American Society for Microbiology (2000).
Chapman, K.B. and Szostak, J.W., “Isolation of a ribozyme with 5′-5′ ligase activity,”Chem. Biol. 2:325-333, Current Biology Ltd. (1995).
Chomczynski P. and Sacchi, N., “Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction,”Anal. Biochem. 162:156-159, Academic Press (1987).
Clark, H.F., et al., “Comparative Characterization of a C-type Virus-Producing Cell line (VSW) and a Virus-Free Cell Line (VH2) FromVipera russelli,” J. Natl. Cancer Inst. 51:645, Oxford University Press (1973).
Dang, Q., et al., “Human Beta Interferon Scaffold Attachment Region Inhibits De Novo Methylation and Confers Long-Term, Copy Number-Dependent Expression to a Retroviral Vector,”J. Virol. 74:2671-8, American Society for Microbiology (2000).
Davis, N.L., et al., “In Vitro Synthesis of Infectious Venezuelan Equine Encephalitis Virus RNA from a cDNA Clone: Analysis of a Viable Deletion Mutant,”Virol. 171:189-204, Academic Press (1989).
Doedens, J., et al., “Inhibition of Endoplasmic Reticulum-to-Golgi Traffic by Poliovirus protein 3A: Genetic and Ultrastructural Analysis,”J. Virol. 71:9054-9064, American Society for Microbiology (1997).
Dryga, S.A., et al., “Identification of Mutations in a Sindbis Virus Variant Able to Establish Persistent Infection in BHK Cells: The Importance of a Mutation in the nsP2 Gene,”Virol. 228:74-83, Academic Press (1997).
Dubensky, Jr., T.W., et al., “Sindbis Virus DNA-Based Expression Vectors: Utility for In Vitro and In Vivo Gene Transfer,”J. Virol. 70:508-519, American Society for Microbiology (1996).
Durocher, Y., et al., “High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells,”Nucleic Acids Res 30:E9, Oxford University Press (Jan. 2002).
Früh, K., et al., “Displacement of housekeeping proteasome subunits by MHC-encoded LMPs: a newly discovered mechanism for modulating the multicatalytic proteinase complex,”EMBO J. 13:3236, Oxford University Press (1994).
Furth, P.A., et al., “Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter,”Proc. Natl. Acad. Sci. USA 91:9302-9306, The National Academy of Sciences (1994).
Garoff, H. and Li, K.-J., “Recent advances in gene expression using alphavirus vectors,”Curr. Opin. Biotechnol. 9:464-469, Current Biology Ltd. (1998).
Gossen, M. and Bujard, H., “Tight control of gene expression in mammalian cells by tetracycline-responsive promoter,”Proc. Natl. Acad. Sci. USA 89:5547-51, The National Academy of Sciences (1992).
Grignani, F., et al., “High-Efficiency Gene Transfer and Selection of Human Hematopoietic Progenitor Cells with a Hybrid EBV/Retroviral Vector Expressing the Green Fluorescence Protein,”Cancer Res 58:14-19, American Association for Cancer Research (1998).
Hennighausen, L., et al., “Conditional Gene Expression in Secretory Tissues and Skin of Transgenic Mice Using the MMTV-LTR and the Tetracycline Responsive System,”J. Cell. Biochem. 59:463-472, Wiley-Liss Inc.(1995).
Herweijer, H., et al., “A Plasmid-Based Self-Amplifying Sindbis Virus Vector,”Hum. Gene Ther. 6:1495-1501, Mary Ann Liebert Inc. (1995).
Hirt, B., “Selective extraction of polyoma DNA from infected mouse cell cultures,”J. Mol. Biol.26:365-369, Academic Press (1967).
Hoffmann, A., et al., “A novel tetracycline-dependent expression vector with low basal expression and potent regulatory properties in various mammalian cell lines,”Nucleic Acids Res. 25:1078-1079, Oxford University Press (1997).
Holowaty, M., et al., “Protein Profiling with Epstein-Barr Nuclear Antigen-1 Reveals an Interaction with the Herpesvirus-associated Ubiquitin-specific Protease HAUSP/USP7,”J Biol Chem. 278:29987-94, The American Society for Biochemistry and Molecular Biology, Inc. (Aug. 2003).
Howe, J.R., et al., “The Responsiveness of a Tetracycline-sensitive Expression System Differs in Different Cell Lines,”J. Biol. Chem. 23:14168-14174, The American Society for Biochemistry and Molecular Biology, Inc. (1995).
Hung, S.C., et al., “Maintenance of Epstein-Barr virus (EBV)oriP-based episomes requires EBV-enco

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