Gene expression regulatory DNA, expression cassette, expression

Details Gene expression regulatory DNA, expression cassette, expression Gene expression regulatory DNA, expression cassette, expression

1997-07-23

1999-09-21

Benzion, Gary

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

536 236, 536 241, 4353201, 435410, C12N 1582, C12N 504, A01H 400

Patent

active

059556498

ABSTRACT:
The present invention provides new gene expression regulatory DNA. This gene expression regulatory DNA comprises a promotor region derived from the barley D-hordein gene enabling the expression of a desired gene, and a regulatory region for regulating the expression of said gene based on said promoter region. This regulatory region consists at least of an activating region to activate the expression of said gene and a suppressing region to suppress the same.
Use of this gene expression regulatory DNA enables controlling as desired the expression of gene within a plant cell such as barley.

REFERENCES:
Sorenson M. et al, "Hordein promoter methylation and transcriptional activity in wild-type and mutant barley endosperm", Molecular and General Genetics, vol. 250, No. 6, Apr. 10, 1996, pp. 750-760.
Cho M. et al, "Expression of hordein promoter-uidA fusions in transgenic barley plants; endosperm-specific B-and D-hordein promoter gene expression in transgenic plant, for potential crop improvement", In Vitro, vol. 32, No. 3, 1996, pp. 103a XP002054427, see abstract.
Thomas M. and Flavell R., "Identification of an ebhancer element for the endosperm-specific expression of high molecular weight glutenin", The Plant Cell, vol. 2, No. 11, Dec. 1990, pp. 1171-1180.
Matzke and Matzke Plant Physiol. 1995. vol. 107: 679-685.
Peltonen et al. Hereditas. 1994. vol. 120:231-239.
Sorenson et al. Mol Gen Genet. 1996 vol. 250:750-760.
Finnegan and McElroy. Bio/Technology. 1994. vol. 12:833-888.
Wan and Lemaux. Plant Physiol. 1994. vol. 104:37-48.

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