Gene expression regulatory DNA

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435 691, 4351723, 435195, 4353201, 536 241, C12N 121, C12N 1511, C12N 1560, C12N 1577

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054398223

ABSTRACT:
The present invention provides a gene expression regulatory DNA and a process for preparing a protein using the same.
A DNA derived from the isocitrate lyase (ICL) gene of a coryneform bacterium regulates expression of a structural gene encoding a protein when incorporated into a vector DNA together with said structural gene and introduced into a host coryneform bacterium, and a useful protein can be efficiently produced using the DNA.

REFERENCES:
patent: 4965197 (1990-10-01), Liebl
Matsuoka, et al. "Isolation, Hyperexpression, and sequencing of the aceA Gene Encoding Isocitrate Lyase in Escherichia coli", J. of Bacteriology 170: 4528-4536, 1988.
Chung et al. "Glyoxylate Bypass Operon of Escherichia coli: Cloning and Determination of the Functional Map", J. Bacteriol. 170: 3861988.
Cadenas et al., Gene 98:117-121 (1991).
Eikmanns et al., Gene 102:93-98 (1991).
Tsuchiya et al., Bio/Technology 6:428-430 (1988).

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