Gene expression modulating element

Details Gene expression modulating element Gene expression modulating element

2007-03-13

2010-02-02

Mehta, Ashwin (Department: 1638)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S320100

Reexamination Certificate

active

07655786

ABSTRACT:
The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

REFERENCES:
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Bevan et al. 2000, Genbank Accession No. AL162971.
Sequence alignment.
Tatiana I. Gerasimova et al., Chromatin Insulators and Boundaries: Effects on Transcription and Nuclear Organization, Annu. Rev. Genet. 2001. 35:193-208.
David A Gdula et al., Genetic and Molecular analysis of the gypsy chromatin insulator of Drosophila, Proc. Natl. Acad. Sci USA, vol. 93 pp. 9378-9383, Sep. 1996.
Wei Wei et al., Polarity of transcriptional enhancement revealed by and insulator element, PNAS, vol. 97 (26) pp. 14518-14523.

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