Gene encoding vitamin b 6 phosphate phosphatase and use...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S117000, C435S122000, C435S320100, C435S252330, C435S254200, C536S023200

Reexamination Certificate

active

07598066

ABSTRACT:
Disclosed is an isolated DNA encoding vitamin B6phosphate phosphatase selected from the group consisting of: (a) a DNA sequence represented in SEQ ID NO:9; (b) a DNA sequence which encodes a polypeptide having vitamin B6phosphate phosphatase activity and hybridizes under standard conditions to the DNA sequence defined in (a) or a fragment of thereof; (c) a DNA sequence which encodes a polypeptide having vitamin B6phosphate phosphatase activity, wherein said polypeptide is at least 70% identical to the amino acid sequence represented in SEQ ID NO:10; (d) a DNA sequence which encodes a polypeptide having vitamin B6phosphate phosphatase activity and is at least 70% identical to the DNA sequence represented in SEQ ID NO:9; (e) a degenerate DNA sequence of any one of (a) to (c).

REFERENCES:
patent: 0 950 715 (1999-10-01), None
patent: WO 03/000875 (2003-01-01), None
Capela et al. GenBank Accession No. Q92SG4, created Dec. 1, 2001.
Jang et al. Human pyridoxal phosphatase. Molecular cloning, functional expression, and tissue distribution. J Biol Chem. Dec 12, 2003;278(50):50040-6. Epub Sep 30, 2003.
Tazoe, M. et al., “Production of Vitamin B6in Rhizobium,” Biosci. Biotechnol. Biochem. v. 63, No. 8, pp. 1378-1382 (1999).
Tazoe, M. et al.,“Biosynthesis of Vitamin B6in Rhizobium,” J. Biol. Chem., v. 275, No. 15, pp. 11300-11305 (2000).
Capela, D. et al. “Analysis of the Chromosome Sequence of the Legume Symbiont Sinorhizobium meliloti Strain 1021,” Proc. Natl. Acad. Sci., v. 98, No. 17, pp. 9877-9882 (2001). EBI Database Abstract No. XP-002275874.
Yang, Y. et al., “Involvement of the gapA- and epd (gapB)-Encoded Dehydrogenases in Pyridoxal 5′-Phosphate Coenzyme Biosynthesis in Escherichia coli K-I2,” J. Bacteriol. , v. 180, No. 16, pp. 4294-4299 (1998).

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