Gene encoding protein capable of regenerating luciferin,...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Reexamination Certificate

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C536S023200, C435S320100, C435S252300, C435S325000, C435S252330

Reexamination Certificate

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06838270

ABSTRACT:
A gene encoding a protein which is capable of regenerating luciferin by acting on oxyluciferin and D-cysteine and thus regenerating luciferin; the above gene originating in a luminous organism; a protein encoded by the above gene; and a process for producing a protein capable of regenerating luciferin characterized by comprising culturing a transformant or a transductant having the above gene transferred therein and collecting the protein capable of regenerating luciferin from the culture. Thus, the protein capable of regenerating luciferin can be efficiently produced, which brings about a great industrial advantage.

REFERENCES:
patent: 5814504 (1998-09-01), Kajiyama
patent: 825257 (1998-02-01), None
patent: 9204468 (1992-03-01), None
N. Shimokawa, et al., Federation of European Biochemical Societies, vol. 327, No. 3, XP-002227524, pp. 251-255, “Molecular Cloning and Sequencing of the cDNA Coding for a Calcium-Binding Protein Regucalcin from Rat Liver”, Aug. 1993.
K. Okada, et al., Tetrahedron Letters, No. 32, XP-002109172, pp. 2771-2774, “Firefly Bioluminescence III. Conversion of Oxyluciferin to Luciferin in Firefly”, 1974.
K. Gomi, et al., The Journal of Biological Chemistry, vol. 276, No. 39, XP-002227525, pp. 36508-36513, “Oxyluciferin, A Luminescence Product of Firefly Luciferase, is Enzymatically Regenerated into Luciferin”, Sep. 28, 2001.
Anil Wipat et al., “The yvsA-yvqA region of the Bacillus subtilis chromosome containing genes involved in metal ion uptake and putative sigma factor”, MICROBIOLOGY, vol. 144, pp. 1593-1600, (1998).

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