Gene encoding novel luciferase

Details Gene encoding novel luciferase Gene encoding novel luciferase

2011-01-18

2011-01-18

Prouty, Rebecca (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S006120, C435S008000, C435S320100, C435S325000, C536S023200

Reexamination Certificate

active

07871803

ABSTRACT:
The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, fromM. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1.MMEIQVLFAL ICFALVQANP TENKDDIDIV GVEGKFGTTD60LETDLFTIVE DMNVISRDTNLANSDADRGK MPGKKLPLEV LIEMEANARK AGCTRGCLIC120LSKIKCTAKM KVYIPGRCHDYGGDKKTGQA GIVGAIVDIP EISGFKELGP MEQFIAQVDL180CADCTTGCLK GLANVKCSALLKKWLPDRCA SFADKIQSEV DNIKGLAGDR210

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Y. Nakajima et al. “cDNA Cloning and Characterization of a Secreted Luciferase from the Luminous Japanese Ostracod,Cypridina noctiluca”, Biosci. Biotechnol. Biochem. 68(3): 565-570 (Mar. 2004).
Markova SV. Et al., Cloning and Expression of cDNA for a Luciferase from the Marine CopepodMetridia longa. J. Biol. Chem., 2004.1, vol. 279, No. 5, pp. 3212 to 3217.
Yasuo Kakunami et al., “Toyamawan ni Okeru KaiashiruiMetridia pacifica(Calanoida) No Biomass Model”, Bulletin of the Japanese Society of Scientific Fisheries, 2000 Nen, pp. 1014 to 1019.

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