Gene encoding mutant L3T4 protein which facilitates HIV infectio

Multicellular living organisms and unmodified parts thereof and – Nonhuman animal

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435455, 435462, 435463, 435 5, 4353201, 435325, 435 691, 424 921, C12N 500, C12N 1500, C12N 1509, C12N 1563

Patent

active

059361387

ABSTRACT:
Disclosed are (1) a DNA sequence encoding a mutant L3T4 protein which, when expressed on the surface of a cell, is capable of facilitating infection of the cell by human immunodeficiency virus; the mutant protein includes at least one amino acid residue substitution or deletion in a segment corresponding to the gp120 binding epitope of a native L3T4 protein so as to increase homology between that segment and its counterpart in a CD4 protein; (2) a murine cell line or strain transfected with such a DNA sequence; and (3) a transgenic mouse susceptible to infection by human immunodeficiency virus.

REFERENCES:
patent: 4873191 (1989-10-01), Wagner et al.
Houdebine, Journal of Biotechnology, vol. 34, pp. 269-287, 1994.
Wall, Theriogenology, vol. 45, pp. 57-68, 1996.
Kappel et al., Current Opinion in Biotechnology, vol. 3, pp. 548-553, 1992.
Strojek & Wagner, Genetic Engineering: Principles and Methods, vol. 10, pp. 221-246, 1988.
Wieder et al., Aids Research and Human Retroviruses, vol. 12, pp. 867-876, Jul. 1, 1996.
Littman et al., Nature, vol. 325, Issued Jan. 29, 1987, pp. 453-455.
Moebius et al., J.Exp.Med., vol. 176, Issued Aug. 1992, pp. 507-517.
Piatier-Tonneau et al., Proc.Natl.Acad.Sci. USA, vol. 88, Issued Aug. 1991, pp. 6858-6862.
Arthos et al., Cell 51 : 469-481 (1989).
L. K. Clayton et al., "Substitution of murine for human CD4 residues identifies amino acids critical for HIV-gp120 binding", Nature, vol. 335, Sep., 1988, pp. 363-366.
P. J. Maddon et al., "The Isolation and Nucleotide Sequence of a cDNA Encoding the T Cell Surface Protein T4: A New Member of the Immunoglobulin Gene Family", Cell, vol. 42, Aug., 1985, pp. 93-104.
S. Ryu et al., "Crystal structure of an HIV-binding recombinant fragment of human CD4", Nature, vol. 348, Nov., 1990, pp. 419-426.
D. Camerini et al., "A CD4 Domain Important for HIV-Mediated Syncytium Formation Lies outside the Virus Binding Site", Cell, vol. 60, Mar., 1990, pp. 747-754.
P. J. Maddon et al., "Structure and expression of the human and mouse T4 genes", Proc. Natl. Acad. Sci., vol. 84, Dec., 1987, pp. 9155-9159.
T. Mizukami et al., Binding reg. for hum. imm. vir. (HIV) and epitopes for HIV-blocking monoclonal anti. of the CD4 molecule defined by site-directed mutagenesis, Pro. Nt. Aca. Sci, vol. 85, 1988, pp. 9273-9277.
B. Tourvielle et al., "Isolation and Sequence of L3T4 Complementary DNA Clones: Expression in T Cells and Brain", Science, vol. 23, Oct., 1986, pp. 610-614.
A. Peterson et al., "Genetic Analysis of Monoclonal Antibody and HIV Binding Sites on the Human Lymphocyte Antigen CD4", Cell, vol. 54, Jul., 1988, pp. 65-72.
Q. J. Sattentau et al., "Structural Analysis Of The Human Immunodeficiency Virus-Binding Domain of CD4", J. Exp. Med., vol. 170, Oct., 1989, pp. 1319-1334.
M. H. Brodsky et al., "Analysis Of The Site in CD4 That Binds To The HIV Envelope Glycoprotein", The Journal of Immunology, vol. 144, No. 8, Apr., 1990, pp. 3078-3086.
J. Arthos et al., "Identification of the Residues in Human CD4 Critical for the Binding of HIV", Cell, vol. 57, May, 1989, pp. 469-480.
P. J. Maddon et al., "The T4 Gene Encodes the AIDS Virus Receptor and Is Expressed in the Immune System and the Brain", Cell, vol. 47, Nov., 1986, pp. 333-348.
N. R. Landau et al., "The envelope glycoprotein of the human immunodeficiency virus binds to the immunoglobulin-like domain of CD4", Nature, vol. 334, Jul., 1988, pp. 159-165.
P. Bedinger et al., "Internalization of the human immunodeficiency virus does not require the cytoplasmic domain of CD4", Nature, vol. 334, Jul. 1988, pp. 162-165.
Littman et al., Nature 325 : 453-455 (1987).
Maddon et al., PNAS 84 : 9155-9159 (1987).
Clayton et al., Nature 335 : 362-366 (1988).
Ryu et al., Nature 348 : 419-425 (1990).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Gene encoding mutant L3T4 protein which facilitates HIV infectio does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Gene encoding mutant L3T4 protein which facilitates HIV infectio, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Gene encoding mutant L3T4 protein which facilitates HIV infectio will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1121697

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.