Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1995-03-28
1995-11-21
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435 691, 435191, 4352523, 4353201, 536 221, 536 231, 536 232, 536 237, C12N 902, C12N 120, C12P 2106, C07H 1900
Patent
active
054686318
ABSTRACT:
The present invention succeeds in isolating a gene encoding an enzyme having an FMN reducing activity and a nitroreductase activity derived from luminous bacteria Vibrio fischeri (ATCC 7744), elucidating its primary structure, and producing Escherichia coli which can express the gene in large quantities. That is, the present invention provides a gene encoding an enzyme having the flavin reducing activity and the nitro-reductase activity, an enzyme produced therefrom, a recombinant vector containing the gene, and bacteria containing the recombinant vector.
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Mutation Research, vol. 216, No. 1, Feb. 1989, NL pp. 211-220 M. Watanabe et al. "A Sensitive method for the detection of mutagenic nitroarenes: construction of nitroreductase-overproducing derivatives of Salmonella typhimurium strains TA98 and TA100".
Archives of Biochemistry and Biophysics vol. 193, No. 1, Mar. 1979, New York, U.S. pp. 110-116, S-C TU et al. "Kinetic studies on the mechanism of bacterial NAD(P)H: flavin oxidoreductase".
Nucleic Acids Research vol. 18, No. 4, 1990, London, GB p. 1059, M. Watanabe et al. "Nucleotide sequence of Salmonella typhirmurium nitroreductase gene".
Inouye Satoshi
Kanoh Hiromasa
Saigo Kaoru
Zenno Shuhei
Chisso Corporation
Kim Hyosuk
Wax Robert A.
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