Gene encoding class I collagenase

Details Gene encoding class I collagenase Gene encoding class I collagenase

1999-08-24

2001-08-28

Achutamurthy, Ponnathapu (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S069100, C435S069700, C435S252300, C435S252330, C435S320100, C536S023200, C536S023400

Reexamination Certificate

active

06280993

ABSTRACT:

BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a gene encoding a class I collagenase, a vector containing the gene, a transformant containing the vector, a class I collagenase, and a method of preparing the class I collagenase.
2. Description of the Related Art
Collagenases belong to a hydrolase, and act on a collagen to decompose a peptide bond. These collagenases play an important role in biotechnology, for example, in a culture of an excised tissue or a cell. In particular, collagenases from Clostridium species are important because they exhibit a reactivity useful in protein engineering. It is known that collagenases from
Clostridium histolyticum
include the class I and class II collagenases, and that the class I collagenase has a high specificity to a naturally occurring collagen and the class II collagenase has a high specificity to a synthesized peptide. Further, a gene encoding the class II collagenase has been cloned.
Nevertheless, the structure of the class I collagenase from
Clostridium histolyticum
is not known, and the gene encoding the class I collagenase from
Clostridium histolyticum
has not been isolated.
SUMMARY OF THE INVENTION
The present invention is based upon an isolation of the gene encoding the class I collagenase from
Clostridium histolyticum.
Accordingly, the object of the present invention is to provide an isolated DNA encoding the class I collagenase. Other objects and advantages will be apparent from the following description.
In accordance with the present invention, there is provided an isolated DNA encoding a class I collagenase, particularly, an isolated DNA encoding a class I collagenase from
Clostridium histolyticum.
Further, in accordance with the present invention, there is provided a vector comprising the isolated DNA encoding the class I collagenase, particularly, the isolated DNA encoding the class I collagenase from
Clostridium histolyticum.
Still further, in accordance with the present invention, there is provided a transformant comprising the vector.
Still further, in accordance with the present invention, there is provided a class I collagenase or a functional equivalent thereof.
Still further, in accordance with the present invention, there is provided a method of preparing a class I collagenase or a functional equivalent thereof by culturing the transformant.


REFERENCES:
patent: WO 94/00580 (1994-01-01), None
patent: WO 98/22574 (1998-05-01), None
Yoshihara et al., 1994, “Cloning and nucleotide sequence analysis of the colH gene from Clostridium histolyticum encoding a collagenase and a gelatinase”, Journal of Bacteriology, vol. 176, pp. 6489-6496.*
Translation of Abstract of Hosaka et al., 1998, “Sequencing of a collagenase gene derived from Clostridium histolyticum, purification of the collagenase , and enzymatic properties thereof”, Seikagaku (Biochemistry), vol. 70, p. 1059, Abstract 4P-259.*
Hosaka et al., 1999, GenBank/EMBL/DDJB nucleotide sequence database Accession No. AB 026889, Clostridium histolyticum genomic DNA segment, 3967 nucleotides, submitted title “Class2 collagenase”.*
Hosaka et al., 1999, GenBank/EMBL/DDJB translated amino acid sequence, SPTREMBL Accession No. Q9SOXO, Clostridium histolyticum protease, 1118 amino acids, submitted title “Class2 collagenase”.*
O. Matsuhita, et al., Journal of Bacteriology, Feb. 1999, vol. 181, pp. 923-933.

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