Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1989-11-30
1992-06-16
Ellis, Joan
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 691, 435 91, 4351723, 4353201, 435255, 435256, 536 27, 530350, 935 13, 935 28, 935 37, 935 56, 935 61, 935 69, C12P 2102, C12P 1934, C12N 1500, C12N 116
Patent
active
051224593
ABSTRACT:
Double-stranded cDNA is prepared from polyadenylated RNA extracted from activated human peripheral blood adherent mononuclear cells. The cDNA is inserted within a plasmid vector and then the recombinant plasmid employed to transform an appropriate host. Transformed hosts are identified and grouped into pools. Plasmid DNA prepared from these pools is hybridized with a labeled, synthetic oligonucleotide probe corresponding to a portion of the amino acid sequence of the interleukin 1 protein. Pools of host cells that provide a positive signal to the probe are identified, plated out and then employed in direct bacterial colony hybridization with the same probe, thereby to isolate the particular positive colony. Plasmid DNA is prepared from this colony and characterized by restriction enzyme mapping and sequencing by chain-termination method. The coding region for the IL-1 gene is inserted into a shuttle vector for amplification of the vector followed by expression of functional IL-1.
REFERENCES:
patent: 4406830 (1983-09-01), Fabricius et al.
patent: 4508833 (1985-04-01), Sonneborn et al.
patent: 4762914 (1988-08-01), Auron et al.
patent: 4766069 (1988-08-01), Auron et al.
patent: 5001057 (1991-03-01), Auron et al.
Roberts et al. (1979) PNAS 76: 760-64.
Windle et al. (1984) J. Immunology 132: 1317-23.
Okayama et al. (1983) Molecular and Cellular Biol. 3: 280-89.
Lachman et al. (1983) FED Proc. vol. 42 pp. 2637-2645.
Clark et al (1984) Proc Nat'l Acad. Sci. USA vol. 81 pp. 2543-2547.
Suggs et al. (1981) Proc. Nat'l Acad. Sci. USA vol. 78 pp. 6613-6617.
Interview with European Patent Office Jun. 13, 1990.
Communication Pursant to Article 96(2) and Rule 51(2) EPC.
Dinarello et al., Proc. Nat'l. Acad. Sci. U.S.A. 74: 4624-27 (1977).
Scala et al., Nature 309: 56-59 (1984).
Letter of Dr. J. Oppenheim dated Sep. 21, 1990.
Auron et al., J. Mol. Cell. Immunol. 2: 169-177 (1985).
Dinarello et al., Rev. Inf. Diseases 6: 51-95 (1984).
Schmidt, J. Exp. Med. 160: 772-87 (1984).
Blyden et al., "Purification and Properties of Human Lymphocyte Activating Factor (LAF)", 118 J. Immunol. 1631-1638 (1977).
Lachman et al., "Partial Purification of Human Lymphocyte-Activating Factor (LAF) by Ultra-filtration and Electrophoretic Techniques", 119 J. Immunol. 2019-2033 (1977).
Togawa et al., "Characterization of Lymphocyte-Activating Factor (LAF) Produced by Human Mononuclear Cells: Biochemical Relationship of . . . ", 122 J. Immunol. 2112-2118 (1979).
Mizel et al., "Characterization of Lymphocyte-Activating Factor (LAF) Produced by a Macrophage Cell Line, P338D.sub.1 ; All . . . ", 120 J. Immunol. 1504-1508 (1978).
Mizel, "Physiochemical Characterization of Lymphocyte-Activating Factor (LAF)", 122 J. Immunol. 2167-2172 (1979).
Mizel et al., "Purification to Apparent Homogeneity of Murine Interleukin Id", 126 J. Immunol. 834-837 (1981).
Lomedico et al., "Cloning and Expression of Murine Interleukin-1 cDNA in Escherichia coli", 312 Nature 458-464 (1984).
Editorial, "Minisymposium on Regulation of Connective Tissue Cells by Immune and Inflammatory Cells", 1 Lymphokine Res. 53 (1982).
Welte et al., "Purification of Human Interleukin 2 to Apparent Homogeneity and its Molecular Heterogeneity", 156 J. Exp. Med. 454-464 (1982).
Acuto et al., "An Efficient Method for Purification of Human T-Cell Growth Factor", 53 J. Immunol. Meth. 15-26 (1982).
Oppenheim et al., "Interleukin 1 is More Than an Interleukin", T-Lymphocytes Today 89-95 (1983).
Kock et al., "Purification of Human Interleukin 1 by High-Performance Liquid Chromatography", 296 J. Chromat. 292-300 (1984).
Hanahan, "Studies of Transformation of Escherichia coli with Plasmids", 166 J. Mol. Biol. 557 (1983).
Stern et al., "Purification to Homogeneity and Partial Characterization of Interleukin 2 from Human T-cell Leukemia", Proc. Nat'l. Acad. Sci. USA 871-875 (1984).
Beggs, "Transformation of yeast by a replicating hybrid plasmid", Nature (London) 104-108 (1974).
March et al., "Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs", Nature 315:641-647 (1985).
G. Di Sabato, "Purification and initial characterization of rat interleukin 2", 79 Proc. Natl. Acad. Sci USA 3020-3023 (1982).
Mosley, et al. Proc. Nat'l Acad. Sci. (USA) 84:45,72, 4576 (1987).
Devereux et al., Nucleic Acid Research, 12: 387-95 (1984).
Andrew C. Webb, et al. "Molecular Organization and Expression of the Prointerleukin-1.beta. Gene" in Recombinant Lymphokines and Their Receptors, (1987), pp. 139-158.
Cerretti Douglas P.
Conlon, III Paul J.
Cosman David J.
Grabstein Kenneth H.
Hopp Thomas P.
Ellis Joan
Immunex Corporation
LandOfFree
Gene encoding biologically active human interleukin 1 does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Gene encoding biologically active human interleukin 1, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Gene encoding biologically active human interleukin 1 will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1753325