Gene encoding asymmetrically active esterase

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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435197, 435 691, 4351723, 4352523, 43525233, 4353201, 536 232, 935 14, 935 29, 935 56, 935 72, 935 73, C12N 1555, C12N 1570, C12N 918, C12P 740

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054057639

ABSTRACT:
An isolated gene encoding an esterase capable of asymmetrically hydrolysing an ester of chrysanthemic acid or its derivative to give an intermediate useful for the production of pharmaceutically and/or agriculturally useful compounds, expression plasmids containing said gene, microorganisms transformed with said expression plasmids, and the production of the esterase by culturing said transformants.

REFERENCES:
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Mitsuda, S. et al. "Microbial Stereoselective Hydrolysis of . . . " Chemical Abstracts 116:12688s (Apr. 1991).
ATCC 1989 Catalog p. 97 entry #53323 Pharmacia 1984 Catalog p. 63.
Deutscher M. (ed). "Guide to Protein Purification" Meth. in Enzymol. vol. 182 pp. 602-613, 738-751 (1990).
Belyavsky, A. et al. "PCR-based cDNA library construction: . . . " Nuc. Acids Res. 17(8) 2919-2932 (Apr. 1989).
Berger, S. L. et al. (eds.) "Guide to Molecular Cloning Techniques". Meth. in Enzymol. vol. 152 pp. 393-399, 415-423, 432-447, 661-704 (1987).
Chemical Abstract, vol. 112, No. 13, 26 Mar. 1990, Sugimoto, M. et al., pp. 610 & JP-A-1 181 788, Jul. 1989.
Chemical Abstract, vol. 118, No. 3, 18 Jan. 1993, Mitsuta, Masaru et al., pp. 583 & JP-A-4 234 991, Aug. 1992.

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