Gene encoding aspartic acid secretory proteinase of Candida albi

Details Gene encoding aspartic acid secretory proteinase of Candida albi Gene encoding aspartic acid secretory proteinase of Candida albi

1993-03-15

1994-07-26

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 2374, 536 232, C12N 1552

Patent

active

053326601

ABSTRACT:
This invention is directed to a gene sequence for secretory aspartic proteinase (SEQ ID No. 1) which is specific to pathogenic Candida yeast, to a highly sensitive method for detection of said gene in a sample solution and a kit used therefor.

REFERENCES:
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"Molecular Cloning", J. Sambrook et al., 1989, Cold Spring Harbor Laboratory Press, p. 8.2.
"Evidence for a role for secreted aspartate proteinase of Candida albicans in vulvovaginal candidiasis", The Journal of Infectious Diseases, vol. 161, No. 6, Jun. 1990, pp. 1276-1283, De Bernardis, F. et al.
"Exponential amplification of nucleic acids: new diagnostics using DNA polymerases and RNA replicases", Trends in Biotechnology, vol. 9, No. 2, Feb. 1991, pp. 53-58, Lizardi, P. M. et al.
Snyder et al. (1987) Methods in Enzymology, vol. 154, ".gamma.gt11: Gene Isolation w/Antibody Probes and Other Applications", pp. 107-128.
Tongi et al. (Jul. 1991) FEBS Lett., vol. 286 (1,2), "Isolation & Nucleotide Sequence of the Extracellular Acid Protease Gene (ACP) from the Yeast Candida tropicalis", pp. 181-185.
Ganesan et al. (Sep. 1991) Infection & Immunity, vol. 59, "Molecular Cloning of the Secretory Acid Proteinase Gene from Candida albicans and Its Use as a Species-Specific Probe" pp. 2972-2977.

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