Gene encoding a regulatory factor involved in activating express

Details Gene encoding a regulatory factor involved in activating express Gene encoding a regulatory factor involved in activating express

1996-07-19

1998-11-03

Railey, II, Johnny F.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 712, 435136, 435169, 4351723, 435227, 4352523, 4353201, 530350, 536 231, 536 2374, C12N 915, C12N 978, C12N 1531, C12P 740

Patent

active

058306935

ABSTRACT:
The invention relates to a regulatory factor substantially containing an amino acid sequence represented by SEQ ID NO:1 and having the action of activating a nitrilase gene promoter, a regulatory factor gene containing DNA coding substantially for said regulatory factor, a recombinant plasmid containing said regulatory factor gene, a nitrilase gene containing a promoter region and a DNA region capable of replicating in a microorganism belonging to the genus Rhodococcus, and a transformant transformed with said recombinant plasmid.

REFERENCES:
patent: 4652639 (1987-03-01), Stabinsky
Komeda et al., Proc. Natl. Acad. Sci. USA 93(20):10572-10577 (1996).
"Nitrilase from Rhodococcus rhodochrous J1" The Journal of Biological Chemistry, vol. 267, No. 29, pp. 20746-20751, Michihiko Kobayashi, et al.
"Transcriptional regulation of the Rhodococcus rhodochrous J1 nitA gene encoding a nitrilase" Proc. Natl. Acad. Sci. USA, vol. 93,pp. 10572-10577, Oct. 1996, Hidenobu Komeda, et al.

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