Gene encoding a polypeptide having nitrile hydratase activity, a

Details Gene encoding a polypeptide having nitrile hydratase activity, a Gene encoding a polypeptide having nitrile hydratase activity, a

1995-06-05

1998-08-04

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing nitrogen-containing organic compound

435232, 4353201, 4352523, 43525411, 536 232, C12P 1302, C12N 100, C12N 988, C12N 1560, C12N 1563

Patent

active

057892113

ABSTRACT:
The present invention relates to a gene derived from Pseudomonas chlororaphis B23 strain which encodes a polypeptide having nitrile hydratase activity being capable of hydrating nitriles to amides. The invention also relates to a recombinant DNA containing the gene, and a transformant transformed with the recombinant DNA. The present invention further relates to a method of producing nitrile hydratase using the transformant and of amides using nitrile hydratase.

REFERENCES:
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Nagasawa et al., 1988, Biochim. Biophys. Res. Commun. 155(2): 1008-1016.
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Abstract entitled "2Xa3 Cloning and Structure Analysis of a Nitrilhydratase (NHase) Gene of Pseudomonas chlororaphis B23" by Nishiyama et al., 1990 (Mar. 1990) 64(03) Nippon Nogeikagaku Kaishi and Applicants' translation thereof.
Abstract entitled "2Xp3 Cloning of Two Types of Nitrilhydratase (NHase) Genes Derived from Rhodococcus rhodochrous " by Kobayshi et al., 1990, (Mar., 1990) 64(03) Nippon Nogeikagaku Kaishi and Applicants' translation thereof.
Ikehata et al., 1988, Lecture Summary, Japan Soc. for Fermentation, p. 22.
Abstract entitled "2Xo2 Cloning of the Amidase Gene of Rhodococcus sp. N-774 and Expression of the Gene in E. coli " by Hashimato et al., 1990 (Mar., 1990) 64(03) Nippon Nogeikagaku Kaishi and Applicants' translation thereof.
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