Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-07
1998-06-09
Walsh, Stephen
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 536 235, C12Q 168, C12P 2100, C12N 1509, C12N 1512
Patent
active
057632162
ABSTRACT:
One shortcoming of methotrexate chemotherapy is that previously responsive tumors can become refractory to methotrexate after continued exposure. Such methotrexate resistance may be due to underexpression of reduced folate carrier (RFC) protein. The present invention provides DNA molecules encoding human RFC. The present invention also relates to expression vectors comprising RFC-encoding DNA molecules, and to the use of such vectors to restore methotrexate sensitivity in mammalian cells. The present invention further relates to antibodies that bind with human RFC protein, and to methods of detecting human RFC protein using such antibodies.
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Cowan Kenneth H.
Dixon Kathy
He Rui
Moscow Jeffrey A.
Lathrop Brian
The United States of America as represented by the Department of
Walsh Stephen
LandOfFree
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