Gene detection method, and intercalator

Details Gene detection method, and intercalator Gene detection method, and intercalator

2005-06-30

2010-11-16

Noguerola, Alex (Department: 1795)

Electrolysis: processes, compositions used therein, and methods

Electrolytic analysis or testing

Involving enzyme or micro-organism

C204S403010, C422S052000, C436S172000, C252S700000

Reexamination Certificate

active

07833406

ABSTRACT:
A gene detection method for detecting a gene having a specific sequence with high sensitivity is provided. The gene detection method comprises a double-stranded nucleic acid formation step of forming double-stranded nucleic acid by hybridizing a nucleic acid probe immobilized on an electrode and a gene sample; a step of adding an intercalator which comprises a compound having a double-stranded nucleic acid binding site Ia that is specifically intercalated into the double-stranded nucleic acid and is covalently bonded to the double-stranded nucleic acid by light irradiation, an electrochemical active site Fa having electrochemical activity, and a connecting site La for connecting the double-stranded nucleic acid binding site Ia and the electrochemical active site Fa, and has an substitution group comprising one of compounds represented by -Lb-Ib and -Lc-Fb or a combination of the both compounds, at each of the respective sites; a light irradiation step of covalently bonding the double-stranded nucleic acid and the intercalator; and a detection step of detecting the intercalator covalently bonded to the double-stranded nucleic acid by electrochemical measurement.

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