Gene detection method

Details Gene detection method Gene detection method

1997-06-30

1999-10-26

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Apparatus

Mutation or genetic engineering apparatus

4352872, C12M 304, C12M 100

Patent

active

059726920

ABSTRACT:
A single stranded nucleic acid probe having a base sequence complementary to the gene to be detected is immobilized onto the surface of an electrode or the tip of an optical fiber, and the nucleic probe is reacted with the gene sample denatured to a single stranded form, and then the nucleic acid probe hybridized with the gene is detected. In this procedure, to the reaction system consisting of the nucleic acid probe and the gene sample, a double stranded nucleic acid recognizing substance capable of binding specifically to the double stranded nucleic acid and being active electrochemically or optically is added. The detection of the nucleic acid probe is conducted by electrochemical or optical determination utilizing the electrode or optical fiber mentioned above. By this method, safer and more convenient detection of the gene is possible at a higher sensitivity even in a reduced time period.

REFERENCES:
patent: 4840893 (1989-06-01), Hill et al.
patent: 5001051 (1991-03-01), Miller et al.
Hashimoto et al., Supramo1. Chem 2(4):265-270, 1993.
Ghosh et al., Nucleic Acid Res 15(13): 5353-5372 (1987) "Covalent Attachment of digonucleotides to said supports".

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