Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2006-02-17
2010-11-23
Woolwine, Samuel (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C536S024300
Reexamination Certificate
active
07838269
ABSTRACT:
Disclosed is a gene detecting method for determining mutation of a specific base or presence/absence of a specific base in a target gene. There are provided a the target gene sample and a control gene sample having a base sequence which is wild-type or standard-type with respect to the target gene. The method comprises steps of (i) independently subjecting the target gene sample and the control gene sample to a PCR reaction for amplification, using primers having an RNA polymerase promoter sequence at the 5′-end thereof, (ii) independently subjecting the double-stranded DNAs produced by said PCR reaction from the target gene sample and from the control gene sample, to an in vitro transcription reaction to form a single-stranded RNA, (iii) independently hybridizing the single-stranded RNAs with a fluorescence-labeled probe composed of a single-stranded DNA having a base sequence complementary to at least part of the base sequence of the control gene and being combined with a fluorescent dye, to form an RNA/DNA hybrid, and then (iv) comparing the fluorescence intensity of the RNA/DNA hybrid derived from the target gene sample with that of the RNA/DNA hybrid derived from the control gene sample.
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Goto Masahiro
Ichinose Hirofumi
Kitaoka Momoko
Okamura Nobuko
Fay Sharpe LLP
Japan Science and Technology Agency
Woolwine Samuel
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