Gene Cry7ba1 encoding an insecticidal crystal protein of...

Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Plant cell or cell line – per se – contains exogenous or...

Reexamination Certificate

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C536S023710, C530S350000, C424S093200

Reexamination Certificate

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07838293

ABSTRACT:
The present invention discloses the isolation, cloning and use of insecticidal crystal proteins fromBacillus thuringiensis. The present invention isolates a novel insecticidal crystal protein gene cry7Ba1 fromB. thuringiensissubsp.huazhongensisYBT-978 strain, and said gene encodes a novel insecticidal crystal protein Cry7Ba1, which shows insecticidal activity against Lepidopteran insects. The present invention also discloses the gene sequence of the novel insecticidal crystal protein, uses suitable expression vectors to transform micro-organisms so as to express the product Cry7Ba1 encoded by the gene and make it exert the insecticidal activity of the protein against Lepidopteran pests.

REFERENCES:
patent: 2008/0070829 (2008-03-01), Carozzi et al.
patent: 12-60397 (2006-07-01), None
patent: WO 93/08693 (1993-05-01), None
patent: WO 95/06730 (1995-03-01), None
Dai et al (1996, Lett. Appl. Microbiol. 22:42-45).
Crickmore et al (1998, Microbiol. Mol. Biol. Rev. 807-813).
Sun Ming, et al.; “Characterization of the Insecticidal Crystal Protein Genes ofBacillus thuringiensisYBT-1520”,Acta Microbiologica Sinica40(4):365-371 (2000).

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