Gene coding for insecticidal crystal protein

Details Gene coding for insecticidal crystal protein Gene coding for insecticidal crystal protein

1986-02-03

1990-04-17

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 43525231, 43525233, 435320, 935 9, 935 23, 935 29, 536 27, C12P 2100, C12N 1500, C07H 1512

Patent

active

049180061

ABSTRACT:
Recombinant plasmids containing the larvicidal delta-endotoxin gene were constructed by inserting HindIII fragments of the Bacillus thuringiensis var. israelensis 72-75 Md plasmid into the Escherichia coli vector pUC12. Two recombinants producing a 27-kdal toxin (pIP173 and pIP174) were indentified by screening clones in an E. coli in vitro transcription-translation system. Both recombinants comprised pUC12 and common 9.7 kb HindIII fragment of the B. thuringiensis plasmid. The 27,340 Da polypeptide synthesized in vito from pIP174 transformed into E. coli JM101 and from B. subtilis 168 and spoOJ87 containing the 1.2 kb TaqI fragment from pIP173 was lethal to mosquito larvae.

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Ward et al. Febs. (1984) vol. 175, #2, pp. 377-382.
Davidson et al. (1984) Curr. Microbiol. 11 pp. 171-174.
Sekar et al. May 1, 1985, vol. 33, #2, pp. 151-158.
Weng et al. J. Biol. Chem. (1983) vol. 258, #2, pp. 1960-1967.

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