Gene and peptide for transcriptional repressor

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se

Reexamination Certificate

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C435S320100, C435S325000, C530S300000, C536S023100

Reexamination Certificate

active

10500361

ABSTRACT:
This invention relates to a peptide or protein capable of converting a transcription factor into a transcriptional repressor, a gene encoding such peptide or protein, a chimeric protein in which the aforementioned peptide or protein is fused to a transcription factor, a chimeric gene in which the gene encoding a peptide or protein is fused to a gene encoding a transcription factor, a recombinant vector having such chimeric gene, and a transformant comprising such recombinant vector. The peptide of the invention that is capable of converting a transcription factor into a transcriptional repressor is very short. Thus, it can be very easily synthesized, and it can effectively and selectively repress the transcription of a specific gene. Accordingly, such gene is applicable to and useful in a wide variety of fields, such as repression of the expression of cancerous genes and regulation of the expression of genes encoding pigment-metabolic enzymes.

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Sato et al. 2000; Structural analysis ofArabidopsis thalianachromosome 3. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and Tac clones. DNA Res. 7: 131-135.
Hiratsu et al. 2004 ; Identification of the minimal repression domain in SUPERMAN shows that the DLELRL hexapeptide is both necessary and sufficient for repression of transcription inArabidopsis.Biochem. Biophys, Res. Commun. 321:172-178.
Masaur Ohta et al., Repression Domains of Class II ERF Transcriptional Repressors Share an Esstential Motif for Active Repression, The Plant Cell, vol. 13, 1959-1968, Aug. 2001, www.plantcell.org@2001American Society of Plant Biologists.
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Hajime Sakai et al., “Role of Superman in maintainingArabidopsisfloral whorl boundaries”, Nature, vol. 378, Nov. 9, 1995, pp. 199-203.
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Masaru Ohta et al., “Three ethylene-responsive transcription factors in tobacco with distinct transactivation functions”, The Plant Journal, (2000) 22(1), pp. 29-38.
Keiichiro Hiratsu et al., “The SUPERMAN protein is an active repressor whose carboxy-terminal repression domain is required for the development of normal flowers”, FEBS Letters 514 (2002) 351-354.
Keiichiro Hiratsu et al., “Identification of the minimal repression domain of SUPERMAN shows that the DLELRL hexapeptide is both necessary and sufficient for repression of transcription inArabidopsis”, Biochemical and Biophysical Research Communications, 321 (2004) pp. 172-178.
Plant and Cell Physiology, vol. 39, no. suppl., May 3, 1998, pp. S63, XP001076676, JP Japanese Society of Plant Physiology, “the whole document”.

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