Gene and gene structure coding for an aminotransferase, and micr

Details Gene and gene structure coding for an aminotransferase, and micr Gene and gene structure coding for an aminotransferase, and micr

1994-11-04

1999-03-30

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

435128, 435 712, 4351723, 4352523, 4353201, 536 232, C12P 1304, C12P 1300, C12N 912, C12N 1554

Patent

active

058887822

ABSTRACT:
A gene and gene structure coding for an aminotransferase, and microorganisms which express this gene.
The preparation of L-2-amino-4-methylphosphinobutyric acid (L-PPT) by transamination of (3-carboxy-3-oxopropyl)-methylphosphinic acid with the aid of the L-PPT-specific transaminase from E. coli DH 1 is very much more efficient when the gene coding for this enzyme is isolated, incorporated into a plasmid and then a microorganism is transformed therewith.

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J. Geisselsoder et al., "Efficient Site-Directed In Vitro Mutgenesis", BioTechniques 5(8) :786-791 (1987).
H. Shiraishi et al., "A rapid and efficient method for targeted random mutagenesis", Gene 64:313-319 (1988).
Wilkinson et al., "A large increase in enzyme-substrate affinity by protein engineering", Nature 307:187-188. (1984).
Smith, "In Vitro Mutagenesis", Ann. Rev. Genet. 19:423-462 (1985).
Zoller and Smith, "Oligonucleotie-directed mutagenesis using M13-dereived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA, Nucleic Acids Research", 10(20) :6487-6500 (1982).

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